摘要
目的研究选择性环氧化酶-2抑制剂塞来昔布联合替吉奥对胃癌裸鼠皮下移植瘤生长的影响及可能机制。方法建立胃癌裸鼠皮下移植瘤模型,成瘤后将裸鼠随机分为阴性对照组、塞来昔布组、替吉奥组、塞来昔布联合替吉奥组,连续给药21 d后,取材,测量肿瘤体积、计算抑瘤率,TUNEL检测肿瘤组织的凋亡率,免疫组化检测各组PCNA、Bcl-2、caspase-3的表达情况。结果塞来昔布组、替吉奥组、联合给药组的抑瘤率分别为30.8%、50.1%、78.8%。各干预组较对照组凋亡率明显增加(P<0.01),联合给药组较单药组凋亡率明显增加(P<0.01)。各干预组PCNA、Bcl-2的表达较对照组明显降低(P<0.01),联合给药组较单药组明显降低(P<0.05)。各干预组caspase-3的表达较对照组明显升高(P<0.05),联合给药组表达较单药组明显升高(P<0.01)。结论塞来昔布、替吉奥均有明显的抗肿瘤作用,二者联合表现为协同作用,可能是抑制肿瘤细胞的增殖、促进凋亡所致。
Objective To investigate the anti-tumor effect of celecoxib combined with tegafur gimeracil oteracil potassium on subcutaneous xenograft tumor of gastric cancer in nude mice and analyze the possible mechanism. Methods A xenograft tumor model of gastric cancer was established subcutaneously in nude mice. After the largest diameter of tumor reached about 5 mm, the nude mice were randomly divided into 4 groups, the control group, the celecoxib group, the tegafur gime- racil oteracil potassium group, and the combination group; the drug was administered respectively for 21 days. Thereafter, tumor tissues were collected, tumor volume was measured, and tumor inhibition rate was calculated. Apoptosis was deter- mined by TUNEL assay and the expression levels of PCNA, Bcl-2 and caspase-3 by immunohistochemistry. Results The tumor inhibition rates of the celecoxib group, the tegafur gimeracil oteracil potassium group, the combination group were 30.8%, .50.1%, 78.8%, respectively. The apoptosis index in treatment groups was higher than that in the control group （P 〈0.0l）, and the combination group was higher than single drug group （P 〈 0.0l）. The expressions of PCNA, Bcl-2 in treatment groups were lower than those in the control group （ P 〈 0.01 ）, and the combination group was lower than single drug group （P〈0.05）. The expression of caspase-3 in treatment groups was higher than that in the control group （P 〈 0.05）, and the combination group was higher than single drug group （ P 〈 0.01 ）. Conclusion Both celecoxib and tegafur gimeracil oteracil potassium showed obvious anti-tumor effect, and the combination of the two acted synergistically. The possible mechanism was that they inhibited tumor growth through inhibiting proliferation and promoting apoptosis of the tumor cells.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2013年第5期458-461,共4页
Chinese Journal of Cellular and Molecular Immunology
