摘要
荧光原位杂交(FISH)技术是一种用荧光素标记的核酸探针和特定片段杂交,通过荧光信号变化对染色体或基因异常进行定性、定位及相对定量分析的方法。淋巴瘤作为常见的恶性肿瘤之一,其分类复杂,不同种类的淋巴瘤常涉及某些特定基因和染色体的改变,并且不同分子特征的淋巴瘤其预后差异也较大。弥漫性大B细胞淋巴瘤(DLBCL)、黏膜相关淋巴组织(MALT)淋巴瘤、滤泡淋巴瘤(FL)、间变性大细胞淋巴瘤(ALCL)等均具有特征性的染色体和基因异常,且不同分子亚型的淋巴瘤也涉及不同的染色体异常。目前FISH技术已被广泛应用于淋巴瘤的分子遗传学研究,并为临床诊断和预后监测提供了较为客观科学的依据。本文对FISH技术在不同类型淋巴瘤分子特征的研究和应用进行综述。
Fluorescence in situ hybridization （FISH） is a cytogenetic technique that detects changes in target genes and chromo- somes using the hybridization of fluorescent-labeled probes to specific DNA fragments on chromosomes. Lymphomas are among the most common malignant tumors worldwide. These tumors have complex subtypes with various responses to therapy and different prog- nosis. The different lymphomas demonstrate abnormal gene and chromosome characteristics, which may be associated with patient prognosis, such as in diffuse large B cell lymphomas, mucosa-associated lymphoid tissue lymphomas, follicular lymphomas, and ana- plastic large cell lymphomas. FISH has been widely used to study the molecular genetics of lymphomas and to provide scientific evi- dence and clues for clinical diagnosis and prognosis. This article gives a brief review of the applications of FISH in the study and diag- nosis of different types of lymphomas.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2013年第10期608-611,共4页
Chinese Journal of Clinical Oncology
基金
国家自然科学基金项目(编号:81071937)
浙江省钱江人才计划项目(编号:2010R10064)资助~~
关键词
荧光原位杂交
淋巴瘤
染色体异常
分子特征
fluorescence in situ hybridization, lymphoma, chromosome abnormality, molecular characteristics
