摘要
利用17个微卫星标记及其荧光标记引物扩增短片段串联重复序列(STR),并通过全自动基因分析仪检测基因型,识别和鉴定了中国荷斯坦种公牛(Bos taurus)的冷冻精液。只允许1个位点有错配作为判别标准,对牛冷冻精液进行个体识别鉴定。结果表明,实验采用的17个微卫星座位的遗传多样性均较高,累积个体识别能力达99.99%,累积偶合率是9.74×10-10,17个微卫星位点适用于牛的个体识别鉴定。
Short tandem repeat(STR)was amplified with fluorescence labeled primers of 17 microsatellite loci and Genetic Analyzer(ABI 3130xl)was used to separate and analyze the genotypes of the amplifiied products.The individual identification of Bulls(Bos taurus)was conducted with the standard that only one locus mismatch was permitted.The results showed that all the 17 microsatellite loci had high genetic polymorphisms,the probability of matching was9.74×10^(-10),and the cumulate discrimination power was 99.99%.This study suggests that the 17 microsate^lites may be used in the bull individual identification.
作者
李倩倩
李莲
李慧
蒋小强
陆汉希
王根林
LI Qian-Qian;LI Lian;LI Hui;LU Han-Xi;WANG Gen-Lin;JIANG Xiao-Qiang(College of Animal Science and Technology,Nanjing Agriculural University,Nanjing 210095;Bovine Frozen Semen Quality Supervision Testing Centre,Agricultural Ministry of China,Nanjing 210095,China)
出处
《动物学杂志》
CAS
CSCD
北大核心
2013年第3期371-376,共6页
Chinese Journal of Zoology
基金
"十二五"国家科技计划课题(No.2011BAD28B02,2012BAD12B10)
江苏省农业三新工程课题(No.SX2011-189
SXGC[2012]394)
关键词
种公牛
微卫星
个体识别
Bull(Bos taurus)
Microsatellite
Individual identification
