摘要
目的:研究从娑罗子中提取娑罗子油的超临界CO2最优萃取工艺及成分分析。方法:以娑罗子油收率为评价指标,采用正交设计试验法优化超临界CO2萃取娑罗子油的工艺参数,并采用GC-MS对娑罗子油成分进行成分分析。结果:超临界CO2萃取娑罗子油的最佳工艺条件为:萃取釜压力28 MPa、釜温度38℃,分离釜Ⅰ压力12 MPa、温度40℃,分离釜Ⅱ压力5 MPa、温度40℃,萃取时间110 min。娑罗子油平均收率为1.264%。对娑罗子油进行了GC-MS分析,共鉴定了26种化合物,主要是脂肪酸成分。结论:与石油醚法相比,超临界CO2萃取具有收率高、萃取时间短、脂肪油比较澄明等优点。对于两种提取方法,油中的含量较高的脂肪酸成分基本一致,含量较低的脂肪酸成分有差别。
Objective：To research the optimal extraction process of supercritical CO2 extraction and analyze the component of the oil extracted from Aesculus wilsonii seed.Methods： Using the yield of Aesculus wilsonii seed oil as the index,optimized supercritical CO2 extraction parameter by orthogonal experiment methodology and analysed the compounds of Aesculus wilsonii seed oil by GC-MS.Results： The optimal parameters of the supercritical CO2 extraction of the oil extracted from Aesculus wilsoniit seed were determined： the extraction pressure was 28 MPa and the temperature was 38 ℃,the separationⅠ pressure was 12 MPa and the temperature was 40 ℃,the separationⅡ pressure was 5 MPa and the temperature was 40 ℃,the extraction time was 110 min.The average extraction rate of Aesculus wilsonii seed oil was 1.264%.26 kinds of compounds were identified by GC-MS in Aesculus wilsonii seed oil extracted by supercritical CO2.The main components were fatty acids.Conclusion：Comparing with the petroleum ether extraction,the supercritical CO2 extraction has higher extraction rate,shorter extraction time,more clarity oil.The kinds of fatty acids with high amounts in Aesculus wilsonii seed oil is identical in general,the kinds of fatty acids with low amounts in Aesculus wilsonii seed oil have differences.
出处
《中药材》
CAS
CSCD
北大核心
2013年第3期475-478,共4页
Journal of Chinese Medicinal Materials
关键词
娑罗子油
超临界CO2萃取
GC-MS分析
Aesculus wilsonii seed oil
Supercritical CO2 extraction
GC-MS analysis
