摘要
目的探讨不同体质SD大鼠急性局灶性脑缺血再灌注后血清中VEGF的表达。方法构建寒、热、常体等3中不同体质的SD大鼠模型,给予急性局灶性脑缺血再灌注损伤,采用酶联免疫吸附法(ELISA)检测不同体质大鼠脑缺血再灌注24h后血清中VEFG的表达情况。结果不同体质大鼠在脑缺血再灌注24h后血清中VEGF均有表达,3种体质大鼠VEFG表达与假手术组比较,C、D两组差异明显(P<0.05),寒体质大鼠血中VEGF表达与其他两种体质大鼠比较,血清中VEGF表达水平低(P<0.05)。结论不同体质SD大鼠脑缺血再灌注损伤后,血清中VEGF均有表达,寒体组VEFG表达水平明显低于其他体质组,说明寒体组大鼠预后较差。
Objective To investigate the different physical SD rats acute focal cerebral ischemia reperfusion serum VEGF expression. Methods Cold, Heat, Normal body 3 different physical build SD rat model, given the acute focal cerebral ischemia-reperfusion injury, enzyme-linked immunosorbent assay （ELISA） to detect different constitution rat brain ischemia after 24h of reperfusion the serum VEFG the expression. Results Physique rats 24h after reperfusion serum VEGF expression in cerebral ischemia, three kinds the physique expression of rat VEFG in comparison with the sham group differences are significant （P〈0.05）, cold constitution rats blood in the expression of VEGF and The other two physical rats, serum VEGF levels （P〈0.05）. Conclusion The physique SD rat brain after ischemia-reperfusion injury, serum VEGF expression, the cold body the group VEFG expression levels were significantly lower than that of other physical group, cold body of rats with poor prognosis.
出处
《中国医药指南》
2013年第10期51-53,共3页
Guide of China Medicine
