摘要
背景:骨髓间充质干细胞与细胞载体联合移植治疗中枢神经系统损伤还处于实验研究阶段。目的:评价大鼠骨髓间充质干细胞与脱细胞脑组织支架的生物相容性,探讨脱细胞脑组织支架作为中枢神经系统组织工程材料的可行性。方法:以全骨髓法分离纯化大鼠骨髓间充质干细胞,通过物理及化学方法相结合制备脱细胞脑组织支架。将转染携带绿色荧光蛋白基因的骨髓间充质干细胞种植到支架材料上共培养,通过倒置相差显微镜、扫描电镜、激光共聚焦显微镜等方法观察脱细胞脑组织支架的内部结构及复合支架上细胞的生长状况。结果与结论:制备的脱细胞脑组织支架材料呈三维立体网状结构。骨髓间充质干细胞可在支架上黏附生长,形态良好。大鼠骨髓间充质干细胞与脱细胞脑组织支架具有良好的生物相容性,有望作为中枢神经系统组织工程的载体材料。
BACKGROUND： Use of scaffolds loaded with bone marrow mesenchymal stem cells for treatment of central nervous system injury is still in the stage of laboratory study. OBJECTIVE： To evaluate the biocompatibility of acellular brain tissue scaffold with rat bone marrow mesenchymal stem cells in vitro and to testify whether the material could be used as a carrier for central nervous system tissue engineering. METHODS： Bone marrow mesenchymal stem cells were isolated and purified by whole bone marrow method, and the acellular brain tissue scaffold was made by both physical and chemical extractionmethods. Bone marrow mesenchymal stem cells transfected by green fluorescent protein were cultured and compounded onto acellular brain tissue scaffold in vitro. The microstructure of the materials and the morphological changes of bone marrow mesenchymal stem ce^ls compounded onto the acellular brain tissue scaffold were observed under inverted phase microscope, scanning electron microscope, and confocal laser scanning microscope. RESULTS AND CONCLUSION： The acellular brain tissue scaffold had the structure of three-dimensional network. The cells grew in an adherent way on the acellular brain tissue scaffold and had normal shape in vitro. The rat acellular brain tissue scaffold has good biocompatibility with bone marrow mesenchymal stem cells and might be used as a biological material for central nervous system tissue engineering.
出处
《中国组织工程研究》
CAS
CSCD
2013年第14期2495-2500,共6页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金资助(30672152)~~
关键词
干细胞
骨髓干细胞
骨髓间充质干细胞
脱细胞
支架
三维立体
网状结构
生物相容性
组织工程
脑组织
绿色荧光蛋白
生物活性
国家自然科学基金
干细胞图片文章
stem cells
bone marrow-derived stem cells
bone marrow mesenchymal stem cells
aceliular
scaffold
three-dimensional
network
biocompatibility
tissue engineering
brain tissue
green fluorescent proteinbioactivity
National Natural Science Foundation of China
stem cell photographs-containing paper
