摘要
目的研究c-Met在子宫内膜腺癌中的表达及其与临床病理参数的关系。方法采用免疫组化和实时荧光定量PCR方法分别检测60例子宫内膜腺癌组织和20例正常子内膜组织中c-Met蛋白和c-Met mRNA的表达。结果 c-Met蛋白的表达与子宫内膜腺癌的组织学分级以及肌层浸润深度有关(P<0.05),与年龄、绝经、临床分期、淋巴结转移无关(P>0.05)。c-Met mRNA在癌组织中的表达量明显高于其在正常子宫内膜组织中的表达量,差异有统计学意义(P<0.01);并且与子宫内膜腺癌组织的分化程度有关(P=0.017)。结论 c-Met的高表达在子宫内膜腺癌的发生发展中起重要作用。
Objective To study the expression of c - Met in endometrial adenocarcinoma（EC） and its relationship with clinical pathological pamameters. Methods The expression of c - Met protein and c - Met mRNA were detected in 60 endometrial adenocarcinoma tissues and 20 normal endometrium tissues by immunohistochemistry and real - time quantitative reverse transcription - polymerase chain reaction （ QRT - PCR） respectively. Results In endometrial adenocarcinoma, the expression of c - Met protein was correlated with histological grade and invasion of deep muscular layer（ P 〈 O. 05 ） , but not with age, pausimenia, clinical stage and lymph node metastasis （P 〉 0. 05）. The positive expression of c - Met mRNA in endometrial adenocarcinoma was higher than that in normal endometrium tissues,and the difference was statistically significant（P 〈 0.01 ）. Moreover,it was correlated with differentiation of endometrial adenocarcinoma （ P = 0. 017 ）. Conclusion The high expression of of c - Met plays an important role in the occurrence and development of endometrial adenocarcinoma.
出处
《医学研究杂志》
2013年第5期90-93,共4页
Journal of Medical Research
基金
浙江省医药卫生科技计划项目(2010KYA175)
关键词
c—Met
子宫内膜腺癌
免疫组化
实时荧光定量
PCR
c - Met
Endometrioid adenocarcinoma
Immunohistochemistry
Real - time quantitative reverse transcription - polymer- ase chain reaction （ QRT - PCR）
