摘要
目的 :研究步长脑心通对脑缺血再灌注(IR)损伤的作用及与ASIC1a的关系。方法:采用ZeaLonga改良线栓法制备大鼠短暂性大脑中动脉栓塞(tMCAO)模型,分为假手术组、MCAO组和脑心通组,分别于再灌注后1、3和7 d用神经系统疾病严重程度评分(NSS)评估神经功能,红四氮唑(TTC)染色法测定梗死体积,干湿重法测定脑含水量,伊文思蓝(EB)法测定血脑屏障通透性,末端脱氧核苷酸转移酶介导的duTP缺口标记(TUNEL)法测定梗死周边凋亡细胞数量,Western blot法测定梗死周边ASIC1a的表达。结果:步长脑心通可显著改善大鼠神经功能缺损,减少梗死体积,降低脑含水量和血脑屏障通透性,减少梗死周边凋亡细胞数量;步长脑心通可显著降低ASIC1a的表达。结论:步长脑心通可通过下调ASIC1a的表达,对局灶性缺血再灌注脑组织发挥保护作用。
Objective： To study the effect of Buchang Naoxintong on the injury of cerebral ischemia re- perfusion and the relation with ASICla. Methods： The models of transient middle cerebral artery occlusion （tMCAO） were established with the Zea-longa modified suture method. Rats were divided into Sham operation group, MCAO group and Naoxintong group. The neural function was evaluated at 1 d, 3 d and 7 d after re- perfusion using Neurological Severity Score. The cerebral infarct volume was measured using TYc-dying method. The water volume in the brain was measured with dry and wet weight method. The cerebral capillary permeability was measured with EB method. Cell apoptosis was detected with TUNEL method and the protein expressions of ASICIa in ischemic brain tissue was detected with Western blotting. Results： Buchang Naoxintong could signifi- cantly improve the neurological deficits of the rats, reduce the cerebral infarct volume, lower the brain water content and the capillary permeability as well as the cell apoptosis. Byuchang Naoxintong was able to greatly abate the expression of ASICIa. Conclusion： Buchang Naoxintong has protective effect on brain tissues with re- perfusion of focal cerebral ischemia by down-grading the expression of ASIC1a.
出处
《温州医学院学报》
CAS
2013年第4期219-222,227,共5页
Journal of Wenzhou Medical College
基金
国家自然科学基金资助项目(81171129)
