摘要
目的探讨Graves病患者外周血辅助性T细胞(Th)17的变化及其意义。方法采用流式细胞仪检测29例新诊断但未治疗的Graves病(GD)患者和21例正常对照者外周血Th17细胞占CD4+T细胞的比例,ELISA法检测Th17相关细胞因子白介素(IL)-17的表达。结果 GD患者外周血Th17/CD4+T细胞的比例和IL-17的表达水平均明显高于正常对照组(P<0.01);Th17细胞比例与IL-17水平呈显著正相关(r=0.612,P<0.01);Th17细胞比例与GD患者血清游离三碘甲腺原氨酸(FT3)、游离甲状腺素(FT4)水平呈正相关(r=0.401,r=0.467,P<0.05),与促甲状腺激素(TSH)水平呈负相关(r=-0.376,P<0.05)。结论 Th17细胞可能参与Graves病的发病。
Objective To investigate the change and its significance of T help cells （Th） 17 in patients with Graves disease. Methods 29 patients with Graves disease （GD） and 21 healthy subjects were selected, all the patients were newly diagnosed and untreated. The frequencies of Th17 in peripheral blood were detected by flow cytometric analysis, and the expression of interleukin（IL）-17 was measured by enzyme-linked immunosorbent assay. Results Compared with the healthy control group, the frequencies of Th17 in peripheral blood and the expression of IL-17 were found to be significantly higher in patients with GD （ P 〈 0. 01 ） ; the proportion of Th17 in peripheral blood had a positive correlation to the level of Th17 （r=0. 612 ,P 〈0.01 ）. Th17 had positive relationship with the level of free triiodothyronine（ FTz ）, free thyroxine （ FY4 ） （ r = 0. 401, r = 0. 467, P 〈 0.05 ） and negative relationship with thyroid stimulating hormone（TSH） （r =0. 376,P 〈0. 05） in GD. Conclusion Th17 cells may involve in the pathogenesis of Graves disease.
出处
《安徽医科大学学报》
CAS
北大核心
2013年第6期682-684,共3页
Acta Universitatis Medicinalis Anhui
