摘要
目的了解宁波地区致泻性病原菌组成和主要流行株的基因分型,探讨菌株的同源性,为追踪传染源提供依据。方法病原菌检测采用直接与增菌分离相结合的方法;细菌鉴定采用生化筛检和API等法;细菌分型采用诊断血清和PFGE分型。结果本组9 256份样本检出8类16种3 473株致泻性病原菌,检出率为37.52%,以副溶血性弧菌最高,与其他病原菌比差异有统计学意义(P<0.05);血清分型发现副溶血性弧菌的O3群、沙门菌的甲型副伤寒、志贺菌的褔氏志贺菌、致病性大肠埃希菌O119为各病原菌的优势菌;PFGE分型副溶血性弧菌分出19个,伤寒沙门菌分出3个,甲型副伤寒沙门菌分出12个。结论副溶血性弧菌是引起宁波地区感染性腹泻最主要的流行株,PFGE分型能显示菌株间的亲缘关系,PFGE分子分型可分析菌株的来源,为流行病学追踪传染源提供依据。
Objective To understand the epidemiological features of diarrheal pathogens, and to subtype the major endemic strains as well as to discuss their homology. Methods Pathogenic bacteria were detected by the combination of direct and enrichment separation method. Biochemical screening and API method were used for the bacterial identification, and PFGE and diagnostic sera were applied for the subtyping of bacteria. Re- sults Out of 9256 samples, 3473 strains were detected with the positive rate of 37.52%, which were classi- fied as 16 species of 8 types. Vibrio parahaemolyticus was found to be the most common bacteria and had higher positive rate than other pathogens detected in samples （P〈0.05） . The serotyping result showed that Vibrio parahaemolyticus 03, Salmonella paratyphi A, Shigella flexneri and Pathogenic Escherichia coli Oll 9 were the dominant prevalent strains among each pathogenic bacteria species. There were 19 Vibrio parahaemolyticus, 3 Salmonella typhi and 12 Salmonella paratyphi A subtyped by PFGE Conclusions Vibrio parahaemolyticus is the major prevalent diarrheal strain in Ningbo. PFGE analysis can aid in epidemiological investigation which help tracking the origin of pathogenic bacteria and uncovering the relationship.
出处
《中国预防医学杂志》
CAS
2013年第5期357-361,共5页
Chinese Preventive Medicine
基金
宁波市自然基金项目(2009A610121)
关键词
感染性腹泻
分离鉴定
病原菌种类
基因分型
Infectious diarrhea
Isolation and identification
Pathogenic bacteria typing
