摘要
目的建立HPLC法测定心脑欣滴丸中总黄酮含量的方法。方法采用Wondasil C18(4.6×250mm,5um)色谱柱,以甲醇-0.4%磷酸水为流动相进行梯度洗脱,检测波长360nm;柱温25℃;流速为1mL·min-1。结果槲皮素、山柰酚、异鼠李素的线性范围分别为0.465～14.88μg·mL-1(R2=1),0.905-28.96μg·mL-1(R2=1),0.30875～9.88μg·mL-1(R2=1);平均加样回收率分别为99.98%(RSD=3.3%,n=6),102.74%(RSD=3.6%,n=6),101.64%(RSD=2.7%,n=6)。结论该方法简单、准确度高、重复性好可用于测定心脑欣滴丸中总黄酮的含量。
OBJECTIVE To develop the method for the determination of total flavonoids in Xinnaoxin dropping pills by HPLC.METHODS The Wondasil C18 column（4.6×250mm,5μm） was used at 25℃ with the linear gradient solvent system consisted of methanol-0.4% phosphoric acid.The detection wavelength was 360nm and the flow rate was 1mL·min-1.RESULTS The linear range of quercetin,kaempferol and isorhamnetin were 0.465-14.88μg·mL-1（R2=1）,0.905-28.96μg·mL-1（R2=1）,0.30875-9.88μg·mL-1（R2=1）,respectively;the average recoveries were 99.98%（RSD=3.3%,n=6）,102.74%（RSD=3.6%,n=6）,101.64%（RSD=2.7%,n=6）,respectively.CONCLUSION The method is simple,accurate and reproducible for determination of the content of total flavonoids in Xinnaoxin dropping pills.
出处
《海峡药学》
2013年第4期29-31,共3页
Strait Pharmaceutical Journal
关键词
HPLC
心脑欣滴丸
总黄酮
HPLC
Xinnaoxin dropping pills
total flavonoids
