摘要
目的探讨早期干预对仔鼠脑组织NB-3的表达及其运动功能的影响.方法孕17~18 d Wistar大鼠45只腹腔注射脂多糖（LPS）380μg/kg连续2 d （LPS组）,另15只注射同等剂量的无菌生理盐水（NS组）.随机选取LPS组仔鼠80只,分为干预组40只（I组）和非干预组40只（NI组）;随机选取NS组仔鼠40只为对照组.早期干预措施主要给予早期触摸与丰富康复训练.仔鼠出生后24 h内在LPS组和NS组随机取5只行脑组织HE染色.其余大鼠分别于出生后14 d、28 d行悬吊试验,1 d、7 d、14 d、21 d、28 d行脑组织免疫组织化学染色,观察NB-3的表达.结果脑组织NB-3的表达及悬吊试验评分三组间均有非常高度显著性差异（P〈0.001）.结论宫内感染可致脑损伤鼠NB-3表达增加,早期干预使脑损伤鼠NB-3表达持续增加,并改善其运动功能.
Objective To investigate the effect of early intervention on adhesion molecule NB-3 expressing in cerebral injured neonatal rat induced with intrauterine infection. Methods Pregnant rats were consecutively injected with lipopolysaccharide （LPS） 380 μg/kg or sa-line 17 d after gestation. The offspring from the LPS group accepted early intervention （I group, n=40） or not （NI group, n=40）, and those for saline group were as control group （n=40）. They were assessed with suspension test 14 d and 28 d after born, and the expression of were NB-3 were detected with immunohistochemistry 1 d, 7 d, 14 d, 21 d, 28 d after born. Results The expression of NB-3 was different among these 3 groups, as well as the scores of suspension test （P〈0.001）. Conclusion Intrauterine infection increases the expression of NB-3 of neo-natal rats with brain damage. The early intervention may increase persistently the expression of NB-3, and improve the motor function.
出处
《中国康复理论与实践》
CSCD
北大核心
2013年第5期436-439,共4页
Chinese Journal of Rehabilitation Theory and Practice
关键词
宫内感染
脑损伤
早期干预
NB-3
神经可塑性
大鼠
intrauterine infection
cerebral injury
early intervention
NB-3
neuronal plasticity
rats
