摘要
目的:观察活血解毒方对糖尿病大鼠胰岛β细胞的保护作用。方法:雄性SD大鼠分为正常组,模型组,活血解毒方高、低剂量组。链脲佐菌素(STZ)腹腔注射65mg/kg造模,放免法检测血清C肽,HE染色观察胰岛,免疫组化法观察胰岛素的表达,TUNEL法检测胰岛细胞凋亡,免疫组化法检测胰岛中核因子-κB(NF-κB)的表达。结果:与正常组比较,模型组大鼠血糖升高(P<0.05),血清C肽含量降低(P<0.01),胰岛萎缩,胰岛内胰岛素表达减少(P<0.05),凋亡增加(P<0.05),NF-κB表达增加(P<0.01)。与模型组比较,活血解毒方低剂量组血糖降低(P<0.05),活血解毒方高、低剂量组大鼠C肽含量升高(P<0.01),胰岛内胰岛素表达增加(P<0.01,P<0.05),凋亡减少(P<0.01),NF-κB表达减少(P<0.01)。结论:活血解毒方可能是通过抑制细胞凋亡及胰岛中NF-κB的表达保护β细胞,促进C肽分泌。
Objective： To investigate the protective effect of Huoxue Jiedu Formula on β cell in pancreatic islet of diabetic rats. Methods： Male SD rats were divided into the normal group, model group, Huoxue Jiedu Formula high dose group, Huoxue Jiedu Formula low dose group. The diabetic rats were established by streptozocin injected intraperitoneally, after four weeks the blood glucose was detected, and the diabetic rats were divided into different groups according to the blood glucose and body weight. The C peptide was detected by radioimmunoassay, the pancreatic islet was observed using HE staining and immunohistochemistry, the apoptosis rate was detected by TUNEL, the level of NF-κB was assessed by immunohistochemistry. Results： In the model group, the glucose in serum increased （P〈0.05）, the C peptide decreased （P〈0.01）, the pancreatic islet of diabetic rats atrophied, a disorganization of cells in pancreatic islet was observed, the insulin reduced （P〈0.05）, the apoptosis rate in pancreatic islet increased （P〈0.05）, the expression of NF-κB increased （P〈0.01）. In Huoxue Jiedu Formula group, the C peptide increased （P〈0.01）, the disorganization of cells in pancreatic islet improved, the insulin elevated （P〈0.05, P〈0.01）, the apoptosis rate in pancreatic islet decreased （P〈0.01）, the expression of NF-κB decreased （P〈0.01）. Conclusion： The Huoxue Jiedu Formula protects β cell in pancreatic islet by inhibiting apoptosis and expression of NF-κB.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2013年第6期1681-1684,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金(No.30901939)
国际科技合作项目(No.2008DFA30610)~~
