摘要
本研究根据RNA干扰的机制原理,利用已构建的包含SP基因部分片段反向重复序列和生物安全性更高的标记基因bar基因的RNAi双元表达载体pDTRSV-IRSP-Bar,通过采用农杆菌介导的方法转化水稻愈伤组织获得抗条纹叶枯病和抗除草剂的转基因水稻植株,通过Basta抗性筛选、PCR检测及Northernblot分析,获得了稳定高抗RSV和抗Basta的株系KRSV1和KRSV9,并对其田间抗性及农艺性状表现进行了调查研究。
According to the principle of RNA interference mechanism,a new RNAi binary vector(named pDTRSV-IRSP-Bar),including inverted-repeat sequence of partial fragment of SP gene and the marker gene bar with higher biosecurity,was used to transform rice calli(CV.Shengdao 13) mediated by Agrobacterium.And transgenic rice plants,KRSV1 and KRSV9,with high RSV-and herbicide-resistance were obtained by Basta screening,PCR detection and northern blotting analysis.The field resistance and agronomy traits were also investigated.
出处
《山东农业科学》
2013年第5期38-41,共4页
Shandong Agricultural Sciences
基金
国家转基因生物新品种培育科技重大专项(2011ZX08001-002)
关键词
水稻条纹病毒
RNA干扰
反向重复序列
农杆菌介导
Rice stripe virus
RNA interference
Inverted-repeat sequence
Agrobacterium-mediated
