摘要
目的:建立丁磺氨酸(BSO)及其胶囊的含量测定方法。方法:以2，4二硝基氟苯为柱前衍生化试剂，反相高效液相色谱法，十八烷基硅烷键合硅胶为填充剂，乙腈0.05 mol·L-1醋酸钠(15∶85，pH6.3)为流动相，检测波长为360 nm。结果:测定胶囊的平均回收率为99.7%(RSD=0.41%，n=5)。结论:本实验建立的HPLC可用于测定BSO及其胶囊的含量。
OBJECTIVE：To determine buthionine sulfoximine (BSO) and its capsules. METHODS：An RP HPLC with 2,4 dinitroflurobenzene pre column drivati zation was developed. The derivatized BSO were separated on an actyldecyl column using a mobile phase of acetonitrile 0.05 mol·L-1 sodium acetate solution (15∶85,pH6.3) with detection at 360nm.RESULTS：The average recovery for determination of BSO capsules was 99.7% ( RSD =0.41%,n=5). CONCLUSIONS：The method could be used to quantitate BSO and its capsules.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2000年第9期533-534,共2页
Chinese Journal of Hospital Pharmacy
关键词
丁磺氨酸
高效液相色谱法
柱前衍生化
胶囊
buthionine sulfoximine (BSO)
HPLC
2,4 dintrofluorobenzene
precolumn derivatization
