摘要
目的 探讨碱性成纤维细胞生长因子 (b FGF)在后发性白内障形成过程中的作用。方法 应用体外培养人的晶状体上皮细胞 ,第 3代细胞悬液接种于 2 4孔培养板内 ,加入不同终浓度的b FGF(0 ,1.0 ,10 .0 ,10 0 .0ng/ml) ,每组重复 3孔 ,培养 72h后 ,细胞计数。结果 b FGF添加组 ,细胞增生呈浓度依赖性 ,b FGF浓度越高 ,细胞增生越快。结论 白内障手术后房水中内源性b FGF的增加可能是后发性白内障的原因之一。
Objective To study the role of b FGF in pathogenesis of after cataract or posterior capsular opacification. Methods The 3rd passage cultured human lensepithelial cells were incubuted in the tissue culturing plates,different concentration of b FGF were added,inclucing 0,1.0, 10.0, 100.0 ng/ml,each group was threeplited.After 72 hours,cell number was measured. Results The more concentration of b FGF,the more the cell proliferation. Conclusion The endogenous b FGF in the aqueous houmour increases after ECCE or phacoemulsification may be the one of the causes of after cataract.
出处
《眼外伤职业眼病杂志》
北大核心
2000年第5期498-500,共3页
Journal of Injuries and Occupational Diseases of the Eye with Ophthalmic Surgeries
基金
山东省教委科研基金资助基金编号J96K17
