摘要
采用杯碟法和生长速率法,对橡胶树枯草芽孢杆菌菌株Czk1发酵液中活性物质进行了初步定位。结果表明:活性物质属于胞外分泌型代谢产物,存在于发酵上清液中。理化性质的研究表明,其抗菌物质表现出耐高温,不耐强酸碱,对紫外线和蛋白酶K不敏感,以及对5种常见的金属离子溶液、有机溶剂具有不敏感的特性;采用酸沉淀后甲醇抽提的方法可提取该活性物质。Czk1培养条件的单因子实验结果表明,YPD培养基作为该菌株的发酵液培养基,初始pH值为7.0,以7%接种量接种,摇床培养(28℃,180r·min-1)3d,Czk1表现出对病原菌最佳的拮抗活性。
Cup-plate methods and plate growth rate method were used to determine antimicrobial substances of strain Czk1 of Bacillus substilis in the fermentation broth. The results indicated that the active component of this strain were extracellular metabolites and existed in the supernatant. Physicochemical analysis showed this antimicrobial component was not tolerant to high temperature and high and low acidity,and not sensitive to ultraviolet radiation,protease K,5 common metal ions and organic solvent. This component could be extracted by acid precipitation and methanol extraction. Single factor experiment showed this strain had the highest pathogen antagonistic activity when shake cultured at 28 ℃ and 180 r·min-1 for 3d on YPD medium inoculated at the rate of 7% and the initial pH7.
出处
《热带生物学报》
2013年第1期36-44,55,共10页
Journal of Tropical Biology
基金
国家天然橡胶产业技术体系病虫害防控专家岗位项目(nycytx-34-GW2-4-3
CARS-34-GW8)
中央级公益性科研院所基本科研业务费专项(2012hzsJ021)
海南大学"211工程"建设项目
