摘要
目的 克隆出血性大肠杆菌O15 7:H7的VT2毒素基因。方法 利用PCR技术从出血性大肠杆菌O15 7:H7染色体基因组中扩增出VT2毒素基因 ,并连接至pGEM -T载体上 ,构建重组质粒pGVT2并进行序列测定。结果与结论 酶切分析表明重组质粒含有VT2毒素基因 ,核苷酸序列分析表明 ,其序列与GenBank上O15
Aim\ To obtain the VT2 toxin gene from the EHEC O157:H7. Method \ By PCR technique, the VT2 toxin gene were amplified from genomic DNA of EHEC O157:H7 strain. And the 1 5kb PCR product was ligated to pGEM-T vector. Result and Conclusion\ Through restriction endonuclease analysis the recombinant pGVT2 clone contained the 1 5kb inserted fragment. And Nucleotide sequence showed that amplified fragment is similar with the sequence of O157:H7 VT2 in GenBank
出处
《中国人兽共患病杂志》
CSCD
北大核心
2000年第5期53-54,31,共3页
Chinese Journal of Zoonoses
