摘要
目的 建立血清P5 3蛋白IgG抗体 (P5 3 IgG)酶免疫测定法 ,初步探讨其临床价值。方法 以P5 3MoAb为包被抗体 ,以亲和层析纯化浓缩P5 3蛋白作为中介 ,自行建立了血清P5 3 IgG酶免疫测定法。结果 78例消化道癌P5 3 IgG阳性率 34.6 2 % (2 7/78) ,而 32名献血员和 30例非癌住院患者均为阴性 ,P均 <0 .0 1。 48例经免疫组化P5 3蛋白染色阳性患者血清P5 3 IgG阳性率 5 6 .2 5 % (2 7/48) ,显著高于阴性对照组 (0 /30 ) ,P <0 .0 1。P5 3 IgG的肿瘤诊断特异性 10 0 % ,敏感度 34.6 2 % ,阳性预测值 10 0 % ,阴性预测值 5 8.82 %。本方法测定特异性 10 0 % ,批内差异 3 .8% ,批间差异 16 .2 %。结论 血清P5 3 IgG测定与P5 3蛋白表达相关 ,是P5 3基因研究的新的辅助指标之一。
Objective To establish an enzyme linked immunosorbent assay (Elisa) method for detecting P53 IgG antibody in serum and to evaluate its preliminary clinical significance. Methods P53 MoAb was used as coating antibody, and the immunopurified P53 antigen through affinity chromatograph acted as an immunosorbent for the serm P53 antibody. We developed an enzyme immunoassay for the detection of P53 IgG antibody.Results P53 IgG antibody was positive in 32.62%(27/78) of the sera from patients with digestive cancer, but in none of 32 healthy donors and 30 non cancer patients ( P <0.01). The positive rate of serum P53 IgG antibody in the positively immunostained cases was 56.26% (27/48), and it was significantly higher than in the negatively immunostained cases (0/30) ( P <0.01). The diagnostic specificity of P53 IgG antibody for the cancer was 100%, the sensitivity was 34. 62%, and the predictive value of positive and negative test was 100% and 58.82% respectively. The specificity of our method was 100%, intraassay coefficient of variation was 3.8%, and the interassay coefficient of variation was 16.2%.Conclusions Serum P53 IgG antibody was correlated to the overexpression of P53 protien in the primary tumor. It was one of auxiliary indicators for investigating the status of the P53 gene in tumor.
出处
《中华检验医学杂志》
CAS
CSCD
2000年第5期291-293,共3页
Chinese Journal of Laboratory Medicine
