摘要
为了研究从川滇无患子果皮中提取无患子总皂苷的工艺条件及总皂苷的生物活性,本文用正交试验的方法研究了总皂苷的提取工艺条件,用药膜法研究了无患子总皂苷提取物的杀虫活性,用MIC方法研究了无患子总皂苷提取物的抗菌活性。川滇无患子果皮中提取无患子总皂苷的最佳工艺条件为提取时乙醇浓度为75%,提取时间为5 h,料液比为1∶5,萃取时正丁醇用量为200 mL。杀虫活性方面研究结果显示与对照组相比无患子总皂苷提取物对杀灭甘蓝蚜、小菜蛾有一定活性,对杀灭甘蓝蚜有一定速效性。其中,无患子总皂苷浓度为5 000 mg/L时对小菜蛾的致死率及化蛹抑制率有较明显的效果,无患子总皂苷浓度为10 000 mg/L时对甘蓝蚜致死作用明显。在抗菌活性方面本研究发现无患子总皂苷提取物的抗菌作用不明显。
To obtain the technology conditions of extracting saponins from Sapindus delavayi pericarp and the bioactivities of saponins, the extraction technology conditions of saponins, insecticidal activity of saponins and antibacteria activity of saponins were studied with the method based on orthogonal tests, sensitive layer and MIC method, respectively. The results showed that alcohol 75% for 5 h, ra tio of solvent to material 1 ： 5, and 200 mL nbutyl alcohol in extraction were the optimal conditions. Compared with the contrast group, the saponins from S. delavayi had a good lethalityrate to Plutella xylostella and Brevicorvne brassicae and could kill B. brassicae rapidly. The effect of lethalityrate and inhibitory action to P. xylostella with 5000 mg/L concentration of S. delavayi saponins was visible and the effect of lethalityrate to B. brassicae with 10 000 mg/L concentration of S. delavayi saponins was visible. In the same time, the saponins didn＇t have visible antibacteria activity.
出处
《云南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2013年第3期433-437,共5页
Journal of Yunnan Agricultural University:Natural Science
关键词
川滇无患子
皂苷
提取工艺
杀虫作用
抗菌活性
Sapindus delavayi
saponins
extracting process
insecticidal activity
anti-bacteria ac-tivity
