摘要
目的 探讨酒精发育毒性和致畸机制。方法 于体外施与孕 9 5d大鼠胚胎不同剂量酒精 ,应用植入后全胚胎培养和荧光偏振技术研究酒精对胚胎发育影响及酒精对卵黄囊 (VYS)细胞膜脂质流动性影响。结果 40 0mg/L酒精对胚胎发育、器官形态分化各指标及VYS细胞膜脂质荧光偏振度 (Pr)和流动度 (LFU)与对照组比较 ,差异无显著性 ;10 0 0mg/L组脑、VYS直径 ,VYS循环、神经管、头长、心脏、DNA含量和Pr显著低于对照组 ,而LFU显著高于对照组 ;2 0 0 0mg/L以上组胚胎发育、形态分化指标显著低于对照组 ,LFU显著增高 ,表明VYS细胞膜脂质流动性增大。结论 酒精具有发育毒性和致畸性作用的靶器官组织是脑和VYS ,酒精发育毒性与其损坏VYS细胞膜影响VYS流动性有关。
Objective To study the mechanisms of developmental toxicity in animals induced by ethyl al cohol. Method Rat embryos of 9 5 days of gestation were exposed to di fferent doses of ethyl alcohol in vitro. Postimplantation whole embryo culture a nd fluorescence polarization technique were used to investigate the effects of a lcohol on embryo development and visceral yolk sac (VYS) membrane lip id fluidity. Result 400 mg alcoho/L caused no significant differences to emb ryo development, organ morphological scores, polarization (Pr value) and lipid f luidity unit (LFU), as compared with the non-alcohol treated controls. 1 000 mg alcohol/L led to the decrease of scores of brain, VYS circle, neurotube, head le ngth and heart, as well as DNA and Pr value; whereas LFU values were increased s igni ficantly. Above 2 000 mg alcohol/L, all indexes were decreased except LFU of VYS cell membrane. Alcohol significantly elevated lipid fluidity of VYS cell membra ne. Conclusions Alcohol causes development toxicity and teratog enisis in rats. Brain and VYS are the targets of alcohol effect. Its development toxicity is related with lipid fluidity and the VYS membrane damage.
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2000年第5期300-302,共3页
Chinese Journal of Preventive Medicine
基金
四川省计划生育委员会资助!(96 0 0 7)
关键词
胎儿发育
卵黄囊
畸形
酒精综合征
Ethanol； Fetal development； Yolk sac； Abnormaliti4
