摘要
为探讨花生在不同硫素水平下氮素代谢特点,选用606为试验材料,硫素设四个水平,测定不同生育时期主要氮代谢酶(硝酸还原酶(NR)、谷氨酰胺合成酶(GS)、谷氨酸合成酶(GOGAT)和谷氨酸脱氢酶(GDH))活性和各器官蛋白质含量。结果表明,施用硫肥显著提高花生不同生育时期叶片中NR、GS、GOGAT和GDH活性;显著增加花生营养器官中蛋白质积累量和转移量,提高籽仁中蛋白质含量。其中施硫量为40kg/hm2时,氮代谢酶活性最大,籽仁蛋白质含量最高。
The experiment aimed at study on the nitrogen metabolism under different levels of sul- fur applying to peanut. 606 was selected as the test material, 4 different levels of sulfur was set to de- termined the main nitrogen metabolism enzyme, nitrate reductase (NR), glutamine synthetase (GS), glutamate synthase (GOGAT) and glutamate dehydrogenase (GDH), activity in different growth stage and the protein content in each organ. The application of sulfur significantly improved the en- zyme activity of NR,GS,GOGAT and GDH in different growth stage. The application of sulfur fertil- izer could increase the uptake and transfer of protein in the vegetative organs and improved the protein content in the seed kernel. The sulfur level of 40 kg/hm2 had the best effect.
出处
《花生学报》
2013年第1期12-17,共6页
Journal of Peanut Science
基金
国家"十一五"科技支撑计划项目(2009BADA8B03)
国家自然科学基金资助项目(30840056)
教育部博士点基金资助项目(20093702110007)
山东省现代花生产业技术体系专项资助
关键词
花生
硫肥
氮代谢酶
蛋白质
peanut
sulfur fertilizer
nitrogen metabolism enzymes
protein