摘要
目的探讨神经突触核蛋白-γ （SNCG）在人脑胶质瘤组织中的表达情况。方法针对43例人脑胶质瘤组织标本，采用逆转录聚合酶链反应（RT—PCR）法测定SNCGmRNA的转录水平，同时采用免疫组化sP法检测SNCG的蛋白表达。结果高级别胶质瘤（HGG，Ⅲ-Ⅳ级）和低级别胶质瘤（LGG，Ⅰ-Ⅱ级）中的SNCG阳性率分别为89．3％（25／28）和73．3％（11／15），胶质瘤病理分级越高SNCG的阳性率越高（χ2=15．724，P=0．000）。SNCGmRNA在HGG和LGG中的转录水平较正常脑组织分别升高（4．46±0．39）倍和（2．56±0．34）倍（P〈0．05），而且HGG中的表达较LGG显著升高（P〈0．05）。结论SNCG的转录和蛋白表达水平在神经胶质瘤中显著增高，检测SNCG表达水平有利于胶质瘤的恶性程度判断。
Objective To explore the expression and significance of synuclein-γ （SNCG） in human gliomas. Methods The expression of SNCG protein was detected by SP immuno - histochemistry and its mRNA was examined by reverse transcription polymerase chain reaction （ RT - PCR） in 43 human gliomas specimens, respectively. Results The positive rates of SNCG in high grade gliomas （ HGG, Ⅲ and Ⅳ） and low grade gliomas （ LGG, Ⅰ and Ⅱ） were 89. 3 % （25/28） and 73.3 % （ 11/15 ）, respectively. The expression of SNCG was associated with the clinical stage （X2 = 15. 724, P =0. 000）. The expression of SNCG mRNA transcription in HGG was higher than LGG （ P 〈 0.05 ） with （2. 56± 0. 34） folds, and higher than normal （ P 〈0. 05） with （4. 46 ±0. 39）folds, and that in HGG was higher than LGG （ P 〈0. 05）. Conclusion The expression of SNCG protein and the level of mRNA transcription increased significantly in gliomas. Detecting SNCG protein or mRNA in human glioma can be used for judging the malignancy degree.
出处
《中华神经外科杂志》
CSCD
北大核心
2013年第5期469-472,共4页
Chinese Journal of Neurosurgery
基金
武警后勤学院院级重点项目（WJM201005,WYM201109）
