rhAng-1通过PKCα/MLC通路影响局灶性脑缺血再灌注大鼠血脑屏障通透性被引量：1

The influence of rhAng-1 on BBB permeability via PKCα/ MLC in focal cerebral ischemia/reperfusion in rats

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摘要目的研究重组人血管生成素1(rhAng-1)对局灶性脑缺血再灌注大鼠血脑屏障内皮细胞骨架的影响。方法大鼠随机分8组:(1)假手术组;(2)缺血组;(3-5)生理盐水+缺血再灌注12h/48h/7d组;(6-8)Ang-1+缺血再灌注12h/48h/7d组。用伊文思兰渗透性实验检测血脑屏障通透性;Westernblot法检测微血管内皮细胞PKCα和p-MLC的表达。结果 Ang-1可明显降低局灶性脑缺血再灌注大鼠血脑屏障通透性,可使脑微血管内皮细胞PKCα和p-MLC表达水平减少。结论 Ang-1降低血脑屏障通透性与降低PKCα/MLC通路表达相关。 Objective This study was performed to determine whether the cerebral microvacular endothelium cytoskeleton was influenced by angiopoietin-l（Ang-1） in focal cerebral ischemia and reperfusion rats. Methods The rats were divided into eight groups randomly：（1） sham-operated group, （2） ischemia group, （3-5） ischemia-reperfusion （middle cerebral artery occlusion and reperfusion （MCAO/R） 12h, 48h, and 7days） and 0.9% saline groups, （6-8） ischemia-reperfusion （MCAO/R 12h, 48h, and 7days） and Ang-1 groups. The BBB permeability was assessed by Evans blue extravasation, the expressions of PKCα and p-MLC were determined by Western blot. Results The BBB permeability of the cerebral microvacular endothelium was significantly decreased after Ang-1 injection, the expression levels of PKC ct and p-MLC in the cerebral microvaeular endothelium were decreased after Ang-1 injection. Conclusion The decrease of the permeability of BBB by Ang-1 may be related to the decrease of PKC α/MLC expression in MCAO/R rats.

作者于航贾庆波王晓艳薛一雪

机构地区哈尔滨医科大学大庆校区基础医学院生理学教研室哈尔滨医科大学附属第五医院胸外科中国医科大学基础医学院神经生物学教研室

出处《解剖科学进展》 CAS 2013年第3期255-258,共4页 Progress of Anatomical Sciences

基金黑龙江省教育厅科学技术研究项目(No.12511189)

关键词重组人血管生成素-1 局灶性脑缺血再灌注 PKCΑ MLC Angiopoietin-1 focal cerebral ischemia/reperfusion PKCα MLC

分类号 R743.33 [医药卫生—神经病学与精神病学]

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