摘要
目的探讨表没食子儿茶素没食子酸酯(EGCG)对刀豆蛋白A(ConA)诱导的小鼠肝损伤的保护作用及其作用机制。方法 40只小鼠随机分为对照组、EGCG对照组、ConA模型组及EGCG+ConA组。采用ELISA法检测血清ALT、AST、TNF-α、IFN-γ、IL-4及IL-6的变化,HE法检测小鼠肝脏形态学改变。结果 ConA模型组小鼠血清转氨酶水平明显高于对照组(P<0.05),EGCG对照组与对照组比较无变化(P>0.05)。与ConA模型组相比,EGCG+ConA组小鼠血清转氨酶水平下降(P<0.05)。ConA模型组小鼠肝脏病理切片示较多肝细胞坏死,对照组和EGCG对照组小鼠肝脏形态学基本正常,EGCG+ConA组示肝细胞炎症坏死程度较ConA模型组有所减轻。ConA模型组小鼠血清TNF-α、IFN-γ、IL-4及IL-6水平明显高于对照组(P<0.05),EGCG对照组与对照组比较无明显差异(P>0.05)。EGCG+ConA组小鼠血清TNF-α、IFN-γ、IL-4及IL-6水平均下降,与ConA模型组比较有显著性差异(P<0.05)。结论 EGCG对ConA所致小鼠肝损伤有明显的保护作用,可能与其对炎性因子的调节有关。
Objective To explore the protective effect and its mechanism of Epigallocatechin-3-gallate（EGCG） in the liver injury of mice caused by ConA. Methods 40 mice were randomly divided into four groups： control group, EGCG group, ConA group, ConA＋EGCG group. The liver injury was examined by measuring serum transaminase, liver histology（HE）, and the TNF-α, IFN-r, IL-4 and IL-6 levels in serum were detected by enzyme linked immunosorbent assay（ELISA）. Results Serum transaminase levels were higher in ConA group than in normal group and EGCG group （P〈0.05） , but decreased in ConA＋ EGCG than in ConA, EGCG also reduced the incidence of liver necrosis. The levels of cytokines were significantly higher in ConA group than in normal group and EGCG group （P〈0.05） , EGCG suppressed inflammatory cytokines TNF-α, IFN-r, IL-4 and IL-6 production as compared with the ConA group （P〈0,05） Conclusion The protective effect of EGCG on hepatic impairment might be related to the inhibition of the inflammatory cytokines.
出处
《解剖科学进展》
CAS
2013年第3期236-238,242,共4页
Progress of Anatomical Sciences
基金
辽宁省教育厅项目(No.2010122)
