摘要
运用傅里叶变换红外光谱技术研究了正常组织(30例)和细胞株(SW 620),正常细胞核和癌变细胞核的红外光谱特征,为红外光谱诊断结直肠癌奠定了细胞及亚细胞水平的实验基础。将所得光谱图的峰位及峰强比进行统计分析发现:细胞株(SW 620)和癌变细胞核中谱带(2 925,1 240和1 085cm-1)的峰位均向高波数移动(p<0.05),而谱带1 400cm-1的峰位均向低波数移动(p<0.05);细胞株(SW 620)中峰强比(I1 650/I1 460,I1 400/I1 460,I1 240/I1 460,)较正常组织升高(p<0.05),而I1 740/I1 460则降低(p<0.01)。癌变细胞核中(I1 650/I1 460,I1 400/I1 460,I1 240/I1 460)较正常细胞核亦升高(p<0.05).这些差异可作为区别组织良恶性的指标。
The purpose of this study is to establish the cell and subcellular basis for the diagnosis of colorectal cancer by researching the FTIR（ Fourier transform infrared spectra） of the normal tissue （30 cases） and cell line （SW 620）, normal nuclei and malignant nucleus with Fourier transform infrared spectrum technology. The results of the peak position and the relative intensi- ty obtained by statistical analysis indicate that the bands at 2 925, 1 240 and 1 085 cm 1 shifted toward higher wavenumber （p〈2 0.05） in the FTIR spectra of cell lines （SW 620） and malignant nucleus. The 1 400 cm 1 band shifted toward lower wave number （p〈0.05）. The relative intensity ratios of 11 650/I1460, I1 400/I1460 and I1240/11460 increased in the FTIR spectra of cell lines （SW 620） compared with normal tissue （p〈0. 05）, but inversely, I1 740 /I1 460 decreased significantly （p〈0. 01）. The relative intensity ratios of I1 650/11460, 11400/I1460 and I1240/I1460 also increased in the FTIR spectra of the malignant nucleus. The above statistic differences are the basis for diagnosing colorectum cancer by FTIR spectroscopy.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2013年第6期1496-1500,共5页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金项目(81172362,81101874)
北京市自然科学基金项目(2122059)
中央高校基本科研业务费专项基金(xjj2010011)资助
关键词
细胞株
细胞核
傅里叶变换红外光谱
Cell line
Cell nucleus
Fourier transform infrared spectra
