摘要
临床观察培养的角膜干细胞羊膜移植片治疗角膜缘功能障碍的效果。方法 :人角膜缘干细胞原代培养后接种生长于羊膜上 ,临床选择角膜缘烧伤后 (碱烧伤、酸烧伤、其他化学烧伤等 )角膜新生血管的患者进行干细胞羊膜片移植 ,术后观察角膜上皮、组织浸润和新生血管情况。结果 :术后患者自觉症状轻 ,视力有一定提高 ,角膜缘移植片紧密贴附 ,角膜上皮完整 ,轻度基质炎性浸润 ,浅层新生血管减少 ,深层新生血管充盈减轻 ,睑球粘连得到松解。结论
To observe the effects of human limbal stem cells cultured on huamn amniotic membrane allograft transplanted for treating human limbal dysfunction.Methods:Human limbal stem cells were planted on amniotic membrane after primary culture.Six patients who had serious corneal neovasculization after limbal burn caused by alkali,acid and other chemical burn were selected and transplantated by allograft limbal stem cells.The corneal epithelium,stromal infiltration and neovasculization were assayed by slit lamp microscope after operation.Results:All patients felt better and visual acuity improving a little,graft including amniotic membrane and stem cells firmly attached on limbus,corneal epithelium become smoothly,stromal infiltration decreased,superficial neovasculization reduced significantly and blepharostenosis vanished.Conclusions:Better conditions could be afforded by allograft limbal stem cells and amniotic membrane transplantation treating limbal dysfunction for following keratoplasty.
出处
《中国实用眼科杂志》
CSCD
2000年第9期524-526,共3页
Chinese Journal of Practical Ophthalmology
关键词
人角膜干细胞
羊膜同种异体移植
角膜缘功能障碍
human corneal stem cells in Vitro amniotic membrane allograft transplantation treatment
