摘要
目的比较藻酸双酯钠(Propylene glycol alginate sodium sulfate,PSS)与阿司匹林、氯吡格雷联合用药后抗凝血、抗血小板及对血液流变学的作用。方法120只雄性Wistar大鼠随机分为正常对照组、PSS组、阿司匹林组、氯吡格雷组、阿司匹林+PSS组、氯吡格雷+PSS组。给药14d后,腹主动脉取血,检测比较血栓弹力图(thromboelastography,TEG)、血小板聚集率、血小板黏附率、血沉、红细胞压积、血浆黏度。结果PSS组与空白组比较,血栓弹力图的R值、K值增加,α-Angle、MA、CI值减小,血小板数目降低,血沉、血浆黏度降低;阿司匹林+PSS组与阿司匹林组比较,氯吡格雷+PSS组与氯吡格雷组比较,R值、K值均增加,α-Angle、MA、CI值减小,血小板聚集率降低;红细胞压积、血小板黏附率各组比较差异无统计学意义(具体结果见正文)。结论PSS对凝血因子及纤维蛋白与血小板形成的血凝块有抑制作用,且阿司匹林或氯吡格雷联用PSS后,使综合凝血指数CI进一步降低,具协同作用,并能显著降低血小板聚集率,具相加作用,无血小板数目减少的不良反应。
Objective To compare the effect of Propylene glycol alginate sodium sulfate （PSS） in combina- tion with aspirin or clopidogrel for anticoagulant effect, antiplatelet effect and on the properties of blood rheology. Methods 120 Wistar male rats were randomly divided into control group, aspirin group, clopidogrel group, PSS group, aspirin＋PSS group, clopidogrel＋PSS group. After 14 days drug administration, blood was collected from abdominal artery for TEG （thromboelastography） test. At the same time, platelet-aggregation rate, platelet-adherence rate, erythrocyte sedimentation, hematokrit, and blood viscosity were tested. Results The value of R and K increased, while a-Angle, MA, and CI de- creased in PSS group compared with control group. For aspirin＋ PSS group and clopidogrel＋ PSS group compared with aspirin group and clopidogrel group respectively, R and K increased and a-Angle, MA, CI decreased （F----25.95~82. 83, P=0. 000~0. 012）. The platelet-aggregation rate decreased in aspirin or clopidogrel plus PSS group compared with control group （x2=25. 207, v= 5, P = 0. 000）. Besides the decrease of erythrocyte sedimentation and blood viscosity （F= 10. 72, 6. 77; P = 0. 000, 0. 011）, the platelet count reduced in PSS group compared with control group （F=6. 98, P=0. 009） but platelet-adherence rate and hematokrit had no difference （F=2. 087, 1.26; P=0. 083, 0. 294）. Conclusion PSS inhibited the coagulation factor and clot by fibrin and platelet. Moreover, aspirin or clo- pidogrel plus PSS can decrease CI and platelet-aggregation rate significantly. At the same time the thrombocytopenia induced by PSS didn＇t appear. In conclusion, the therapy by aspirin plus PSS, clopi- dogrel plus PSS may be better choices for anti-coagulant and antiplatelet treatment than aspirin or clopi- dogrel alone.
出处
《中国海洋药物》
CAS
CSCD
北大核心
2013年第3期49-53,共5页
Chinese Journal of Marine Drugs
基金
海洋药物教育部重点实验室(中国海洋大学)开放基金项目(KLMD(OUC)201202)资助
关键词
藻酸双酯钠
联合用药
抗凝血
抗血小板
血液流变学
PSS
combination therapy
anticoagulant effect
antiplatelet effect
blood rheology
