摘要
目的:探索一种快速建立颈动脉粥样硬化动物模型的有效方法。方法:将30只雄性日本大耳白兔随机分为3组,分别给予正常饲料喂养、单纯脂肪乳剂ig和脂肪乳剂ig加氮气损伤(脂肪乳剂按胆固醇∶猪油∶蛋黄粉1∶5∶4配制;同时历时5 min,150 mL.min-1氮气流损伤血管内皮)。于实验第4周和第8周,检测动物血脂含量并观察颈动脉病理变化特征。结果:脂肪乳剂手术组大耳白兔4周时已经形成高脂血症,血清总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)含量分别达(17.55±5.36),(13.68±2.67)mmol.L-1较空白组显著升高(P<0.01),内膜轻度增厚呈现动脉粥样硬化早期改变;8周时TC,LDL-C含量进一步升高分别达(21.13±3.59),(15.18±3.29)mmol.L-1,内膜明显增厚可见大量泡沫细胞及脂质沉积。而上述病理改变未见于空白组、脂肪乳剂组和脂肪乳剂手术组对侧的颈动脉。结论:脂肪乳剂加氮气损伤能有效的建立兔颈动脉粥样硬化模型。
Objective： To establish an experimental animal model of carotid atherosclerotic in rabbit. Method： Thirty male Japanese White rabbits were randomly divided into three groups. The control group fed with normal diet, the fat-emulsion group gavage with high cholesterol fat emulsion, the experimental group gavage with high cholesterol fat emulsion and operated by nitrogen-drying（fat emulsion formulation： cholesterol： pork fat： custard powder 1： 5： 4; nitrogen-drying operation： lasted 5 min and 150 mL·min^-1 airflow rate）. Blood lipids and pathological change examination of carotid artery were done at the 4 and 8th week after surgery. Result： At 4th week later, the content of total cholesterol （TC） and low density lipoprotein （LDL-C） were （17.55±5.36）, （13.68±2.67） mmol·L^-1, which significant higher than blank-control group （P〈0.01）, and the pathological studies suggested that early atheromatosis appeared, such as intimal thickening lightly. At 8th week later, the content of TC and LDL-C were （21.13±3.59）, （15.18±3.29） mmol·L^-1, and the typical carotid atherosclerosis was observed which include significant intimal thickening and secondary vascularization in intimal, the accumulation of lipid and cholesterol in the associated cells in the high cholesterot fat emulation and operated by nitrogen-drying group. No arthersclerosis were seen in the control group, fat-emulsion group and self-control group. Conclusion： The animal model of carotid atherosclerotic in rabbit can be effectively established by fat emulsion plus nitrogen gas injury.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第11期176-179,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
综合性中药新药研究开发技术大平台(2009ZX09301-005)
关键词
颈动脉粥样硬化
高脂血症
脂肪乳剂
模型
carotid artery atherosclerosis
hyperlipidemia
fat-emulsion
model
