摘要
目的:建立高效液相色谱法同时测定糙枝金丝桃药材中绿原酸、芦丁、金丝桃苷、槲皮素、山奈酚的分析方法。方法:用Sunfire C18(4.6 mm×250 mm,5μm)色谱柱分离;乙腈-0.1%磷酸进行梯度洗脱;流速1.0 mL.min-1;检测波长360 nm。结果:绿原酸、芦丁、金丝桃苷、槲皮素、山奈酚分别在0.01～0.61μg(r=0.999 6),0.10～2.02μg(r=0.999 7),0.10～1.01μg(r=0.999 9),0.10～1.24μg(r=0.999 7),0.005 7～0.17μg(r=0.999 7)线性关系良好;平均加样回收率分别为98.87%(RSD 1.43%),99.81%(RSD 0.86%),99.98%(RSD 0.82%),98.8%(RSD 1.73%),99.85(1.79%)。结论:该方法简便、准确,重复性好,可用于糙枝金丝桃药材的质量控制。
Objective： To establish an HPLC method for the simultaneous determination of chlorogenic acid, rutin, hyperoside, quercetin and kaempferol in Hypericum scabrum. Method： A high-performance liquid chromatography equipped a Sunfire C18 （4.6 mm×250 mm, 5 μm） column with UV detector used. The mobile phase consisted of acetonitrile and 0.1% aqueous phosphoric acid with gradient elution. The detection wavelength was set at 360 nm and the flow rate was 1.0 mL·min^-1 Result： The linear ranges of chlorogenic acid, rutin, hyperoside, quercetin and kaempferol were 0.01-0.61 μg （r=0.999 6）, 0.10-2.02 μg （r=0.999 7）, 0.10-1.01 μg （r=0.999 9）, 0.10-1.24 μg （r=0.999 7）, 0.005 7-0.17 μg （r=0.999 7）, the average recoveries were 98.87% （RSD 1.43%）, 99.81% （RSD 0.86%）, 99.98% （RSD 0.82%）, 98.8% （RSD 1.73%）, 99.85 （RSD 1.79%）. Conclusion： The method is simple, accurate and repeatable, which can be used for quality control of H. scabrum.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第11期75-78,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
新疆医科大学科研创新基金项目(XJC201114)
