摘要
目的探讨上皮钙黏蛋白(E-cadherin)和基质金属蛋白酶(MMP-2和MMP-9)在胃癌组织中的表达及临床意义。方法应用免疫组织化学SABC法和Western blot法检测60例原发性胃癌和15例正常胃黏膜组织E-cadherin和MMP-2、MMP-9的表达。结果胃癌组织中E-cadherin的阴性表达率为88.33%,显著高于正常胃黏膜组织的20.00%(P<0.01)。MMP-2和MMP-9在胃癌组织中的阳性表达率分别为67.39%和73.91%,高于正常胃黏膜组织的31.54%和21.47%(P<0.01)。E-cadherin的阴性表达率与胃癌的分化程度、淋巴结转移有关,而与患者年龄、性别、肿瘤大小及临床分期无显著相关性;MMP-2和MMP-9的阳性表达率与E-cadherin的阴性表达率呈正相关(r=0.453和r=0.579,P<0.01)。结论胃癌组织高表达MMP-2和MMP-9,同时低表达E-cadherin,提示E-cadherin和MMP-2、MMP-9的异常表达可能参与了胃癌的发生、发展过程。
Objective To study the expression and significance of E-cadherin, matrix metalloproteinase（MMP）-2 and MMP-9 in gastric carcinoma. Methods The expressions of E-cadherin, MMP-2 and MMP-9 were detected in 60 specimens of gastric carcinoma and 15 normal gastric mucosa tissues by immunohistochemistry（SABC method） and Western blot. Results The percentage of negative expression of E-cadherin in gastric carcinoma was 88. 33 %, which was higher than 20. 00% in normal gastric mucosa （P〈0. 01）. The percentages of positive expressions of MMP-2 and MMP-9 were 67.39% and 73.91% in gastric carcinoma, which were higher than 31.54% and 21.47% in normal gastric mucosa （P〈0, 01 ）. The negative expression rate of E-cadherin was related to differentiation and lymph node metastasis, but not related to the size of tumor, patient＇s age, sexual distinction,and clinical stage. The positive expressions of MMP-2 and MMP-9 in gastric carcinoma were positively correlated to negative expression of E-cadherin（r=0. 453 and r=0. 579, P〈0. 01）. Conclusion The abnormal low expression of E-cadherin and high expressions of MMP-2 and MMP-9 in gastric carcinoma may play an important role in its pathogenesis,infiltration and metastasis.
出处
《江苏医药》
CAS
北大核心
2013年第9期1034-1036,F0003,共4页
Jiangsu Medical Journal
基金
江苏大学高级人才专项基金(1283000352)
关键词
胃癌
上皮钙黏蛋白
基质金属蛋白酶
Gastric carcinoma
E-cadherin
Matrix metalloproteinase
