摘要
应用杂交瘤技木制备抗重组人肿瘤坏死因子-α单克隆抗体对于r-HTNF-α的纯化和TNF-α分子的抗原性及功能的研究将是一种主要工具。本实验对一组抗rHTNF-α单克隆抗体的特性和功能进行了研究。Western blotting结果表明Z_4、Z_8、Z_(12)、Z_ (20)、Z_(21)、B_3和E_6 抗体特异性地识别分子量为17000道尔顿的TNF-α它们与rIL-1、rIL-2、rIFNγ、rIFNα、和E coli菌裂解液无交叉反应。竞争性ELISA结果证实7种抗体分别识别TNF-α分子上5个不同的抗原决定簇。其中4个抗体可以识别TNF-α活性中心部位。两个抗体还可以识别天然TNF-α分子。
Monoclonal antibodies against recombinant human tumor necrosis factor alpha ( TNFα ) were produced by hybridoma techniques. The research on the potential of these antibodies for the purification of TNFα, the development of specific immuno-assays and defining antigentic and functional domains of TNFa was conducted. The results of Wester Blotting showed that Z4. Z8, Z12, Z20, Z21, B3 and E6 antibodies could recognize TNFa of M.Wr 17000 doltons. None of them crossreacted with rH-1, rIL-2, rIFNα, rIFNα1 and E. Col; lysates. Competitive ELISA test demonstrated that seven antibodies recognized respectively five distinct determinants in TNFa. Four antibodies from them could also bind functional centre of TNFα. E6 and Z8 antibodies could recognize nalur TNFα, too.
关键词
肿瘤坏死因子
单克隆抗体
tumor necrosis factor alpha
antibodies, monoclonal
