摘要
建立动物源食品中全氟辛酸(PFOA)和全氟辛烷磺酸(PFOS)的聚酰胺固相萃取法。样品采用酸化乙腈提取,在酸性条件下用聚酰胺固相萃取小柱富集、净化,5%氨水-甲醇溶液洗脱后采用超高效液相色谱-串联四极杆质谱检测。PFOA和PFOS在1～60ng/mL质量浓度范围内均呈良好的线性关系,r=0.9999。精密度在0.8%～6.2%之间(n=5);两种待测组分的检测限均为0.2μg/kg;对6种动物源食品的加标回收率在93.3%～102.8%之间。
A method for the extraction of perfluorooctanoic acid（PFOA） and perfluorooctane sulphonic acid（PFOS） in foods of animal origin using polyamide solid-phase extraction（SPE） was developed.Samples were extracted with acidified acetonitrile,cleaned up and enriched on a polyamide SPE cartridge under acidic conditions through elution with 5% ammonia-methanol and determined by ultra performance liquid chromatography-tandem mass spectrometry（UPLC-MS/ MS）.The calibration curves for PFOA and PEOS exhibited good linearity（r = 0.9999） in the concentration range of 1 to 60 ng/mL,and the detection limit of the developed method was 0.2 μg/kg for both compounds and the average recovery rates were in the range of 93.3% to 102.8% with relative standard deviations（RSD） of 0.8% to 6.2%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第10期241-245,共5页
Food Science
基金
福建省质量技术监督局科技研究项目(FJQI2009023)
关键词
超高效液相色谱-串联四极杆质谱仪
动物源食品
全氟辛酸
全氟辛烷磺酸
聚酰胺固相萃取
ultra performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS）
animal-origin food
perfluorooctanoic acid（PFOA）
perfluorooctane sulphonic acid（PFOS）
polyamide solid-phase extraction
