摘要
研究膨胀床富集与固定床层析去杂偶联优化螺旋藻藻蓝蛋白分离纯化过程的效果。第一步,通过自制的膨胀床(400mm×16mm)对螺旋藻藻蓝蛋白的粗提液进行分离富集,藻蓝蛋白的最高纯度可由粗提液的0.23提高至2.02,得到的藻蓝蛋白质量浓度从粗提液的0.17mg/mL提高为2.15mg/mL,纯度为1.00,藻蓝蛋白回收率为59.45%。第二步,在XAD7HP固定床(200mm×10mm)上对膨胀床纯化后获得的样品进行层析,去除杂蛋白。在优化的条件下,获得的藻蓝蛋白层析样品的最高纯度达到2.20,样品中藻蓝蛋白的质量浓度为0.87mg/mL,藻蓝蛋白回收率为58.81%。整个偶联方式藻蓝蛋白总回收率为34.96%。
Expanded bed enrichment coupled with impurity removal with a packed bed was used for the separation and purification of phycocyanin.Crude phycocyanin extract was adsorbed by a self-made expanded bed（400 mm × 16 mm）.The resulting maximum purity（A620 nm/A280 nm） of phycocyanin was 2.02 compared to 0.23 for the crude extract.It was also found that the phycocyanin concentration of the crude extract was 0.17 mg/mL and increased to 2.15 mg/mL after this treatment,with a purity of 1.00 and a recovery of 59.45%.In the second step,a packed bed of XAD7HP macroporous resin（200 mm × 10 mm） was used to remove impurity proteins from the crudely purified product.The maximum purity of phycocyanin obtained under optimum conditions was 2.20 with a recovery of 58.81%,and the phycocyanin concentration of the purfied product was 0.87 mg/mL.The recovery of phycocyanin from the initial crude extract was 34.96%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第10期107-111,共5页
Food Science
关键词
膨胀床
藻蓝蛋白
分离纯化
偶联
expanded bed
phycocyanin
purification
coupling
