摘要
采用盐酸作为提取剂,超声辅助提取法对羊栖菜中的无机砷进行提取,氢化物发生-原子荧光光谱法(HG-AFS)测定羊栖菜中的无机砷。为了优化羊栖菜中无机砷的提取工艺,在单因素试验基础上,利用Box-Behnken试验设计原理和响应面分析法,以荧光强度为响应值,选取浸提液浓度、超声提取温度和提取时间3个因素,研究各因素及其交互作用对荧光强度的影响。结果显示:无机砷的最佳提取条件为浸提液浓度6.6mol/L、提取温度53.4℃、提取时间24.4min,在此条件下,检测所得荧光强度为675.51,通过计算得无机砷含量为4.78mg/kg。
Ultrasonic-assisted extraction was utilized to extract inorganic arsenic from Sargassum fusiforme（Harv.） by hydrochloric acid.Hydride generation atomic fluorescence spectrometry（HG-AFS） was used for the determination of inorganic arsenic.Based on the single factor tests,the Box-Behnken central composite design and response surface methodology（RSM） were adopted to obtain the optimal conditions for the extraction of inorganic arsenic from Sargassum fusiforme（Harv.）,using fluorescence intensity as the response value and the extraction concentration,extraction temperature and time as the factors.The results showed that the optimum extraction conditions of inorganic arsenic were the extraction concentration 6.6 mol/L,extraction temperature 53.4 ℃ and time 24.4 min.Under these conditions,the fluorescence intensity was 675.51,and the content of inorganic arsenic was 4.78 mg/kg.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第10期67-71,共5页
Food Science
基金
上海市科委工程中心建设项目(11DZ2280300)
关键词
原子荧光法
羊栖菜
无机砷
超声提取
响应面
atomic fluorescence spectrometry（AFS）
Sargassum fusiforme（Harv.）
inorganic arsenic
ultrasonicassisted extraction
response surface methodology
