摘要
目的观察黑米皮提取物对人前列腺癌PC-3细胞增殖的抑制作用并探讨其机制。方法用不同浓度的黑米皮提取物(20,150和300μg/ml)处理PC-3细胞,通过MTT法、流式细胞仪、western blot法研究提取物对细胞的影响。结果处理组的细胞增殖率随着提取物作用浓度和时间的增加而降低(P<0.05);与对照组相比,处理组细胞凋亡率增加,差异有统计学意义(P<0.05);细胞周期中,G1期细胞百分比显著增多,S、G2-M期细胞显著减少,细胞被阻滞在G1-S期。与对照组相比,处理组细胞的p-p38、p-JNK的蛋白表达则随着提取物浓度的增加而增强。结论黑米皮提取物能够抑制人前列腺癌PC-3细胞的增殖,改变细胞周期的分布,激活JNK、p38通路,诱导细胞凋亡。
Objective To observe the inhibitive effects of black rice pericarp extracts on cell proliferation of human prostate cancer cell PC-3 and to explore its effecting mechanism.Methods The black rice pericarp extract was used to treat the PC-3 cells.The inhibitory effect of black rice pericarp extract on cells proliferation of PC-3 was tested by MTT method.Cell apoptosis rates and cell cycle were measured by flow cytometric assay（FCM）.Western blot was used to study the protein expression levels of p38,p-p38,JNK,p-JNK.Results A dose-dependent and time-dependent proliferation inhibition of black rice pericarp extract was demonstrated in PC-3.The most prominent experiment condition was inhibitory concentration with 300μg/ml and treated for 72h.The experiment result of flow cytometry analysis demonstrates that the apoptosis rate of PC-3 cells increased along with the increasing of black rice pericarp extract concentration,and a G1-S cell cycle arrest was induced in a dose-dependent manner.After PC-3 cell was treated with black rice pericarp extract for 72h,the expressions of p-p38,p-JNK protein increased.Conclusion Black rice pericarp extract could inhibit proliferation,change the cell cycle distributions and induce apoptosis in human prostatic cancer cell PC-3.Its inhibitory effect may be through promoting activation of the JNK,p38 signaling pathway.These results suggest that black rice pericarp extract maybe has an inhibitory effect on prostatic cancer.
出处
《卫生研究》
CAS
CSCD
北大核心
2013年第3期474-477,482,共5页
Journal of Hygiene Research
基金
国家自然科学基金资助项目(No.30500407)
