摘要
目的:研究谷维素与黄连素及其配伍在大鼠中药动学的作用规律。方法:18只雌雄各半健康SD大鼠,随机平均分为3组:谷维素组(OZ),黄连素组(BBR)及黄连素及谷维素按1∶1比例混合组(BBR-OZ),各组口服给药量均为100mg.kg-1,给药后在不同时间点采血,样品经处理后采用液质联用法(LC/MS/MS)测定血浆中谷维素主要代谢物阿魏酸(FA)及黄连素(BBR)的浓度。DAS药动学软件拟合房室模型,进行单用谷维素、黄连素及合用时主要药动学参数的比较分析。结果:口服给药后,谷维素及黄连素在大鼠体内符合二室开放模型,在单用和配伍给药后的各药动学参数均具有明显统计学差异。两者配伍给药后,FA分布加快,消除减慢,吸收减慢并显著增加;BBR分布减慢,消除加快,同时吸收减慢并增加。结论:谷维素与黄连素合用后,影响各自的吸收、分布、代谢与消除,具有明显的药动学相互作用。
OBJECTIVE To study the pharmacokinetic mutual influence between γ-oryzanol and berberine.METHODS 18 rats were randomly divided into 3 groups： OZ,BBR and BBR-OZ（BBR∶OZ=1∶1）.After oral administration 100 mg·kg-1 of OZ,BBR and BBR-OZ to rats,blood samples were collected at different time intervals and the concentrations of FA and BBR in the obtained unknown plasma were determined by LC/MS/MS.The pharmacokinetic data were analyzed by Drug Analysis System（DAS） software.RESULTS After oral administration,the disposition process of OZ（FA） and BBR in rats could be described by open two-compartment model.Compared to OZ and BBR group,significant differences were found on the pharmacokinetic parameters of FA and BBR in BBR-OZ group.FA was distributed faster and eliminated slower but the absorption was significantly increased,while BBR was distributed slower and eliminated faster but the absorption was increased.CONCLUSION There is a pharmacokinetic mutual influence between OZ and BBR,which influence the absorption,distribution,metabolism and elimination between them.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2013年第10期793-796,共4页
Chinese Journal of Hospital Pharmacy
关键词
液质联用
阿魏酸
黄连素
药动学
相互影响
LC/MS/MS
ferulic acid（FA）
berberine（BBR）
γ-oryzanol（OZ）
pharmacokinetic
mutual influence
