摘要
目的观察微小RNA-100(miR-100)在胃癌患者血浆中的表达情况,探讨其与临床病理特征之间的关系及临床意义。方法采用实时定量聚合酶链反应检测30例胃癌患者及30例正常成年人血浆中miR-100的表达情况,分析其与临床病理参数之间的相关性。结果胃癌患者血浆中的miR-100表达水平显著高于正常成年人(P<0.05);血浆miR-100表达水平与肿瘤分化程度有关(P<0.05),而与淋巴结转移、分期、性别、年龄无明显相关(P>0.05)。结论血浆miR-100作为新的生物学指标对于胃癌的诊断具有一定的价值。
Objective To observe the expression of microRNA-lO0 （miR-100） in serum of gastric cancer patients, and explore the relation and clinical significance between its expression and its clinicopathologic features. Methods Real time quantitative reverse transcription-pdymerase chain reaction was performed to detect the expression of miR-100 in the serum of 30 patients with gastric cancers and 30 healthy adults. Furthermore, the associations between miR-100 expression in serum of patients and the elinicopathologic features were analyzed. Results The expression level of miR-100 in serum of gastric cancer patients was significantly higher than that of healthy adults（ P 〈 0.05 ）. The expression level of miR-100 in serum of patients was significantly associated with tumor differention （ P 〈 0.05 ）, but it was not correlated with gender, age, clinical stage, and lymph node metastasis status（ P 〉 0.05 ）. Conclusion miR-100 as a new biomarker, has a certain value for the diagnosis of gastric cancer.
出处
《新乡医学院学报》
CAS
2013年第5期384-386,共3页
Journal of Xinxiang Medical University
