摘要
采用响应面法对费氏新萨托菌G5产β-甘露聚糖酶发酵过程进行优化分析。在单因素实验的基础上选取8个因素,采用Plackett-Burman法进行筛选,确定NaCl和CaCl2浓度对β-甘露聚糖酶产量影响较大。用最速上升实验和中心组合实验进一步优化,利用Design-expert软件进行二次回归分析,得到最适宜发酵培养基配比为:瓜尔胶13.0 g/L,蛋白胨11.0 g/L,酵母粉5.0 g/L,MgSO4 1.0 g/L,KH2PO4 0.5 g/L,NaCl 0.6 g/L,CaCl2 0.6 g/L,pH值为4.0,此时酶产量为采用初始培养基发酵时酶产量的2.5倍。
The response surface methodology（RSM） was used to obtain optimal medium components for Neosartorya fischeri G5 β-mannanase production.8 factors were selected for Plackett-Burman design based on single factor experiment to evaluate the influence.The steepest ascent experiment and central composite design were adopted for further optimization.Design-Expert statistic methods were used for quadratic regression model equation to find out the optimum concentration levels and the relationships among factors.The optimal medium consisted of guar gum 13.0 g/L,peptone 11.0 g/L,yeast powder 5.0 g/L,MgSO4 1.0 g/L,KH2PO4 0.5 g/L,NaCl 0.6 g/L and CaCl2 0.6 g/L at pH 4.0,and the final enzyme yield was more than twice of the original.
出处
《化学工业与工程》
CAS
2013年第3期7-12,共6页
Chemical Industry and Engineering
基金
天津市自然科学基金(09JCZDJC26500)
关键词
费氏新萨托菌
Β-甘露聚糖酶
响应面
优化
Neosartorya fischeri
β-mannanase
response surface methodology
optimization
