摘要
一次干燥温度位的高低直接影响一次干燥所需时间.实验研究了干燥箱搁板温度对血小板冷冻干燥保存的影响,检测了冻干血小板复水后的数值恢复率、细胞形态和结构、血小板凋亡和活化等指标,与新鲜组进行了对照分析.结果显示:当搁板温度为-30℃时,血小板冻干复水后的数值恢复率为(89.0±3.2)%;血小板平均体积(MPV)和分布宽度(PDW)值分别为(12.4±1.5)fL和(18.3±1.5)%,在可以接受的范围之内;未凋亡率为(87.5±2.6)%,非活化率为(80.4±2.6)%.
The time duration needed for primary drying is directly related with the temperature level used during the primary drying process. The effects of shelf temperature of the drying cabinet on the lyophilization of human platelets were experimentally studied. The cell recovery rate after rehydration, the morphological and structural changes, the non-apoptosis rate and the non-activation rate were detected and compared with those of fresh set. The results show that when the shelf temperature is set at -30~C, the cell recovery rate after rehydration reaches （89.0 ± 3.2）% and the MPV and PDW were （12.4 ± 1.5）fL and （18.3 ± 1.5） % respectively,both of which are within acceptable ranges. The non-apoptosis rate of rehydrated platelets is （87.5 ± 2.6） % and the non-activation rate is （80.4 ± 2.6） %.
出处
《上海理工大学学报》
CAS
北大核心
2013年第2期187-192,共6页
Journal of University of Shanghai For Science and Technology
基金
中央高校基本科研业务费专项基金资助项目(2012QHA4008)
关键词
血小板
冷冻干燥保存
一次干燥
搁板温度
恢复率
, platelet
freeze drying preservation
primary drying
shelf temperature
recovery rate
