摘要
目的探讨羊水细胞核型分析在妊娠中期胎儿产前诊断中的必要性。方法从2003年3月份至2011年2月份妊娠16-22周具备产前诊断指征的妊娠妇女4703例,在B超引导下进行羊水穿刺取羊水15-20ml,对羊水脱落细胞进行培养,收获,制片,G显带,并进行细胞核型分析。结果在4703例羊水细胞核型分析结果中,共检出异常核型306例,检出率为6.51%。其中染色体数目异常为142例,占3.02%,染色体结构异常为139例,占2.96%,检测到嵌合体23例,占0.49%,标记染色体2例,占0.04%。结论羊水细胞核型分析在产前诊断中可避免染色体患病胎儿的出生,是产前诊断中不可替代的手段。
Objective： To explore the necessity of karyotyping for prenatal cytogenetic diagnosis. Methods： From March 2003 to February 2011, 4703 women with 16 -22 weeks pregnancy were genetically screened for Prenatal diagnosis indications in the Prenatal Diagnosis Center of Shenzhen Maternity and Child Healthcare Hospital. All the patients were given amniocentesis under the guidance of B ultrasound and extracted 15 - 20ml amniotic fluid, Amniotic fluid exfoliated cells were cultured and harvested for G - band karyotype analysis. Results： 306 abnormal karyotypes were detected in all the 4703 women, the ration is 6. 51%. it included 142 Chromosome number abnormalities （3.02%）, 139 Chromosome structural abnormalities （2.96%）, 23 Chimeric chromosomes （0. 49% ）, 2 marker chromosomes （0. 04% ）. Conclusion： Analysis on karyotype of amniotic fluid ceils Can avoid the birth of the fetus chromo- some abnormality, it is a irreplaceable means in prenatal diagnosis.
出处
《中国优生与遗传杂志》
2013年第5期49-50,60,共3页
Chinese Journal of Birth Health & Heredity
关键词
羊水
核型分析
产前诊断
Amniotic fluid
Karyotype
Prenatal diagnosis
