摘要
本试验旨在研究硫胺素对亚急性瘤胃酸中毒(SARA)状态下山羊瘤胃发酵特性的影响。选用6只1～2岁装有永久性瘤胃瘘管、身体健康的徐淮山羊为试验动物,分为试验组和对照组,每组3只,采用逐渐提高饲粮精粗比的方式诱导发生SARA。试验动物处于SARA状态后,试验组饲粮中添加240 mg/kg硫胺素。结果表明:饲粮中添加硫胺素提高了瘤胃液pH;饲粮中添加硫胺素显著或极显著降低了乳酸(4～8 h)和乙酸的浓度(0～12 h)以及乙酸/丙酸(0～12 h)(P<0.05或P<0.01),显著或极显著提高了丙酸(0～12 h)与丁酸的浓度(0、6、9 h)(P<0.05或P<0.01);饲粮中添加硫胺素显著或极显著降低了各时间点组胺浓度(P<0.05或P<0.01),但未使除6 h外的内毒素浓度发生显著变化(P>0.05),显著或极显著提高了各时间点瘤胃液硫胺素浓度(P<0.05或P<0.01),显著或极显著降低了硫胺素酶的活性(0、6、9 h)(P<0.05或P<0.01)。结果提示,饲粮中添加240 mg/kg硫胺素能够改善SARA状态下瘤胃内环境,缓解山羊SARA。
The effects of thiamin on the rumen fermentation characteristics in goats suffered from subacute ru- minal acidosis （SARA） were studied. Six healthy Xuhuai goats aged 1 to 2 years and fixed with permanent fis- tulas were divided into two groups with 3 goats in each group. SARA of goats was induced by a feeding regime of gradual increasing dietary ratio of concentrate to forage. After SARA occurs, 240 mg/kg thiamin was added in the diet of experimental group. The results showed that the supplementation of thiamin increased rumen fluid pH; the concentrations of lactate （4 to 8 h）, acetate （0 to 12 h） and acetate/propionate （0 to 12 h） were sig- nificantly decreased （P 〈0.05 or P 〈0.01 ）, while the concentrations of propionate （0 to 12 h） and butyrate （0, 6 and 9 h） were significantly increased （P 〈0.05 or P 〈0.01 ） ; thiamine concentration in rumen fluid at all the time points was significantly decreased （P 〈 0.05 or P 〈 0.01 ）, while endoxin concentration in rumen fluid was not significantly affected at all the time points excepted for 6 h （ P 〉 0.05 ） ; thiamin concentration in rumen fluid was significantly increased at all the time points （ P 〈 0.05 or P 〈 0.01 ）, while the activity of thi- aminase was significantly decreased （ P 〈 0.05 or P 〈 0.01 ）. It is concluded that the supplementation of 240 mg/kg thiamin in diets for goats during SARA period can improve the conditions of rumen internal envi-ronment, which results in the release of SARA.
出处
《动物营养学报》
CAS
CSCD
北大核心
2013年第5期1004-1009,共6页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
国家自然科学基金资助项目(31072051)
江苏高校优势学科建设工程资助项目
