摘要
随机选取池塘专养(M1)、日本沼虾套养(M2)以及水库放养(M3)三种养殖模式养成的肛长(25.91±3.26)cm的日本鳗鲡(Anguilla japonica)作为研究材料,采用生物化学方法测定并比较了不同养殖模式日本鳗鲡养成品血清生化指标和脏器消化酶与抗氧化酶活力。结果表明:(1)在所测16项血清生化指标中,三者间均无显著差异的为TP、ALB、GLB、ALT和CHE(P>0.05),均具显著差异的为CK、CREA和α-HBDH,它们的测定值依次呈M3>M2>M1(P<0.05)、M3>M1>M2(P<0.05)和M2>M1>M3(P<0.05);(2)所测消化酶中,三者均具显著差异的为肝脏蛋白酶、胃蛋白酶、肝脂肪酶及胰腺脂肪酶,它们的测定值均呈M1>M2>M3(P<0.05);(3)所测抗氧化酶中,三者间均无显著差异的为心脏SOD、CAT、POD和肝脏CAT活力(P>0.05),均具显著差异的仅为肝脏POD活力,其测定值呈M1>M3>M2(P<0.05)。
The （25.91±3.26）cm snout-vent lengths Anguilla japonica were randomly selected as research materials from three cultured population, namely monoculture population （M1）, polyculture population （M2） which were kept in the pounds of Macrobrachium nipponense, and proliferation population （M3） that were collected from huge reservoirs. The biochemical method was used to determine and compare the serum biochemical indices, viscera digestive enzymes activity and antioxidant enzyme activity of three cultured population of A. japonica. The results show that： （1） among the 16 serum biochemical indicators, there were no significant differences in the TP, ALB, GLB, ALT and CHE （P〉0.05） for individuals of these three cultured population. CK, CREA and cz-HBDH were the indicators that showed significant differences, and the measured values revealed that M3 〉 M2 〉 M1 （P〈0.05）, M3 〉 MI 〉 M2 （P〈0.05） and M2 〉 Ml 〉 M3 （P〈0.05）, respectively. （2） Liver protease, pepsin, hepatic lipase and pancreas lipase were the measured digestive enzymes that showed significant differences among individuals of these three cultured population. Their measured values all revealed that M1 〉 M2 〉 M3 （P〈0.05）. （3） The measured antioxidant enzymes that showed no significant differences among individuals of these three cultured population were the SOD, CAT, POD in the heart and CAT in the liver （P〉0.05）. The activity of POD in the liver was the only indicator that showed significant differences among three cultured population, and the measured values re- vealed that Ml 〉 M3 〉 M2 （P〈0.05）.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
2013年第2期403-408,共6页
Oceanologia Et Limnologia Sinica
基金
浙江省重大科技专项农业重点项目
2008C12083号
关键词
日本鳗鲡
养殖模式
血清生化指标
消化酶
抗氧化酶
Anguilla japonica
aquaculture model
serum biochemical indices
digestive enzymes
antioxidant enzymes
