摘要
目的 探讨原发性肝癌患者血清白细胞介素 12 (IL 12 )与外周血和癌组织中T淋巴细胞亚群变化的规律及其相关性。方法 应用ELISA技术检测 36例原发性肝癌患者血清IL 12水平 ,采用流式细胞技术检测外周血T淋巴细胞亚群 ,免疫组化技术检测肝癌组织中T淋巴细胞亚群分布。结果 随着肝癌的发展 ,肿瘤体积增大和癌栓的形成 ,外周血CD+4 细胞数目减少 ,CD+8细胞数目增多 ,T4/T8比值进行性下降。血清IL 12水平和T4/T8比值间存在显著相关性。结论 IL 12是肝癌生物抗肿瘤的重要因素 。
HT6”,5”] [WT5”BZ] [WT5”HZ]Objective [WT5”BZ]To explore the changes and interrelationship of serum interleukin 12 (IL 12) and T lymphocyte subset in patients with primary hepatic carcinoma (PHC). [WT5”HZ]Methods [WT5”BZ]Serum IL 12 level was determined by ELISA in 36 patients with PHC. The peripheral blood T lymphocyte subset was assessed with flow cytometry. The distribution and changes of T lymphocyte subset in the tumor tissue were detected by immunohistochemistry analysis. [WT5”HZ]Results [WT5”BZ]The numbers of the CD + 4 T cell were reduced and of the CD + 8 T cell increased either in peripheral blood or tumor tissue, and showed the trend of the ratio (T4/T8) declined progressively with the aggravation of the state with PHC. IL 12 and T4/T8 had significant interrelationship. [WT5”HZ]Conclusion [WT5”BZ]IL 12 is an important antitumor factor of the patients with PHC. T lymphocyte subset plays a great role in the process of antitumor. [HT6,5”] [WT5”HZ]
出处
《中国普外基础与临床杂志》
CAS
2000年第5期302-304,共3页
Chinese Journal of Bases and Clinics In General Surgery
