摘要
目的 观察深低温保存不同时期人视网膜的酶组织化学及超微结构变化。 方法 以酶组织化学染色法测定深低温保存不同时期 (2 75、70 4d)及对照组视网膜琥珀酸脱氢酶 (succinicdehydrogenase ,SDH)、乳酸脱氢酶 (lacticdehydrogenase ,LDH)、腺苷三磷酸酶 (ATPase)活性 ,并观察视网膜组织结构及超微结构改变。 结果 深低温保存不同时期及对照组的人视网膜SDH、LDH及ATPase活性差异无显著性意义 ;光镜下见深低温保存组视网膜神经纤维层、外网层及杆锥层积液略多 ;透射电镜下神经纤维结构正常 ,细胞核形态、结构正常 ,线粒体不同程度肿胀 ,个别空泡形成。 结论 深低温法是视网膜的活性保存方法之一 ,有望为临床视网膜移植提供材料。
Objective [WT5”BZ]To observe the enzymic histochemical and ultrastructral changes of cryopreserved human retina. [WT5”HZ]Methods [WT5”BZ]To compare the activity of lactate dehydrogenase (LDH), succinate dehydrogenase (SDH) and ATPase in cryopreserved retina with those in fresh retina and to observe the histological and ultrastructural changes of cryopreserved retina. [WT5”HZ]Results [WT5”BZ]There was no statistical difference between the activity of LDH, SDH and ATPase in fresh and in cryopreserved retina. Histologically, in the cryopreserved retina, fluid in neural fiber and outer plexiform layers, as well as in cone and rod layer, was sligthly more than normal. The ultrastructure is normal except that the mitochondria was swollen in different degree. [WT5”HZ]Conclusion [WT5”BZ]Cryopreservation may be an effective method for keeping the retinal cells alive for a long period and might free the transplantation from dependance on aviability of fresh dornor tissue. [WT5”HZ]
出处
《中华眼底病杂志》
CAS
CSCD
北大核心
2000年第3期198-200,共3页
Chinese Journal of Ocular Fundus Diseases
关键词
视网膜移植
超微结构
深低温保存
酶组织化学
Retina/ultrastructure
Cryopreservation
Histology
Tissue transplantation
