摘要
目的：揭示金属蛋白酶类（ＭＭＰｓ）及层粘连蛋白受体（ＬＮ－Ｒ）与人黑色素瘤细胞侵袭转移的关系，并探讨 ＭＭＰｓ及ＬＮ－Ｒ用以判断肿瘤细胞侵袭转移的可能性。方法：通过流式细胞术（ＦＣＭ）定量研究和蛋白酶活性分析 （Ｚｙｍｏｇｒａｐｈｙ），对具有不同潜在转移能力的人黑色素瘤细胞系（ＷＭ３５，ＷＭ１３４１ｂ，ＷＭ９８３ａ，ＷＭ４５１）进行 ＭＭＰｓ及瘤 细胞表面６７ ０００ ＬＮ－Ｒ的荧光阳性率和全部细胞的平均荧光强度测定。结果：早期 ＷＭ３５不产生 ＭＭＰｓ；ＷＭ１３４１ｂ 仅产生ＭＭＰ－２，不产生ＭＭＰ－９；进展期ＷＭ９８３ ａ和远处转移瘤株ＷＭ４５１既产生ＭＭＰ－２又产生 ＭＭＰ－９。瘤细胞表面 ６７ ０００ ＬＮ－Ｒ的荧光阳性率和全部细胞的平均荧光强度大小顺序为：ＷＭ４５１＞ＷＭ９８３ａ＞ＷＭ １３４１ｂ＞ＷＭ３ ５。结论： ＭＭＰｓ和ＬＮ－Ｒ与人黑色素瘤细胞侵袭转移能力的获得之间关系密切，并可作为较特异的肿瘤侵袭转移标记物应用 于肿瘤研究与治疗中。
Aim: To explore the relation between metalloproteinases(MMPs),Laminin receptor(LN-R) and invasive metasta- sis of human melanoma cell and probe their probability of judging tumor invasive metastasis. Methods: Through the quantitative analysis of FCM and Zymography, studied human melanoma cell lines with different metastatic potenials. Results: At early stage, WM35 didn't produce MMPs, and WM134lb produced only MMP-2, but no MMP-9. Both WM983a at progressive stage and WM451 produced MMP-2 and MMP-9.The fluorescent positive the of 6700 0 LN-R on the surface of melanoma cells and the average fluorescent intensity were WM451 > WM983a > WM1341b > WM35. Conclusion: MMPs and LN-R closely correlate with inva- sive metastasis ability of human melanoma cell lines and may be as a specific mark of tumor invasive metastasis when used in study and treatment of tumor.
出处
《河南医科大学学报》
2000年第5期424-426,共3页
Journal of Henan Medical University
