摘要
主要探讨小型工业化水平的超滤膜技术纯化麦芽根中5′-磷酸二酯酶的工艺,通过比较确认,选取截留分子量为13ku的超滤膜浓缩纯化原料液,经优化后其工作参数为:膜运行温度30℃、膜前压0.8MPa、膜表面流量20LPM,此时所得浓缩液中酶比活力为3018.9U/mg,较纯化前提升了21.2倍,酶活力回收率达到了80.8%。证明选用膜技术纯化5′-磷酸二酯酶是完全可行的。
Discusses the process of the 5＇-phosphodiesterase purification in membrane technology of small scale industrialization. Selecting ultrafiltration membrane with molecular weight cut off 13000 to purify the raw feed solution. After optimization, its working parameters are temperature 30℃, membrane pressure 0.8 MPa, membrane surface flow 20 LPM. The enzyme specific activity is 3018.9 U/mg, the purification factor is 21.2 times and the enzymatic activity recovery is 80.8%. The results show that the purification of the 5＇- phosphodiesterase in membrane technology is feasible.
出处
《食品科技》
CAS
北大核心
2013年第5期241-243,249,共4页
Food Science and Technology
关键词
麦芽根
5’-磷酸二酯酶
超滤膜
barley roots
5＇-phosphodiesterase
ultrafiltration membrane
