摘要
[目的]研究不同培养基配方对防止土人参愈伤组织玻璃化的效果。[方法]以MS+6-BA 2.0 mg/L+NAA 0.5 mg/L+蔗糖30g/L为基础培养基(对照组),将其配方作以下分组调整:①6-BA浓度减半;②添加活性炭(1 g/L);③Ca2+加倍;④NH4+减半;⑤采用2,4-D+KT代替6-BA;⑥联合②③④⑤,比较培养基变化对土人参愈伤组织玻璃化率的影响,并观察土人参愈伤组织悬浮培养细胞形态。[结果]与对照组相比,②～④组玻璃化率无显著差异(P>0.05);⑤组玻璃化率降至25%(P<0.05);⑥组玻璃化率降为0,同时⑥组悬浮细胞密度及活性均高于对照组。[结论]最佳培养基为改良MS(Ca2+浓度加倍,NH4+浓度减半)+2,4-D 2.0 mg/L+NAA 2.0 mg/L+KT 0.5 mg/L+活性炭1.0 g/L+蔗糖30 g/L,可在一定程度上防止土人参愈伤组织玻璃化的发生。
[Objective] To investigate the effect of different medium formula on preventing Talinum paniculatum's callus vitrification.[Method] With the MS+6-BA 2.0 mg/L+NAA 0.5 mg/L+ sugar 30 g/L as the basic medium,the following grouping adjustment was treated in the basic medium(control group): ① reduction in concentration by half of 6-BA;② adding activated carbon(1 g/L);③ doubling the concentration of Ca2+;④ reduction in concentration by half of NH4+;⑤ 6-BA replaced by 2,4-D + KT;⑥ combination of ②③④⑤.The effects of different Medium Formula on T.paniculatum's callus vitrification rate were compared and the morphological characters of suspension-culture cells of T.paniculatum callus were observed.[Result] Compared with the control group,there was no significant difference in vitrification rate of group ①-④(P>0.05),while that of group ⑤ was reduced to 25%(P<0.05),and even to 0 for group ⑥.The density and activity of suspension-culture cells of group ⑥ were higher than those of the control group.[Conclusion] The optimal medium,modified MS(doubling the concentration of Ca2+,reduction in concentration by half of NH4+)+ 2,4-D 2.0 mg/L + NAA 2.0 mg/L + KT 0.5 mg/L + activated carbon 1.0 g/L + sugar 30 g/L,could effectively prevent the occurrence of Talinum paniculatum's callus vitrification.
出处
《安徽农业科学》
CAS
2013年第8期3307-3309,共3页
Journal of Anhui Agricultural Sciences
基金
广东药学院2012年省级大学生创新创业训练计划项目(1057312001)
广东药学院2012年校级大学生创新实验项目(14)
关键词
土人参
培养基配方
愈伤组织培养
玻璃化
Talinum paniculatum
Medium formula
Callus culture
Vitrification
