摘要
目的 通过对浙江者兰溪市某污染区儿童δ氨基 γ 酮戊酸脱氢酶 (ALAD)基因的遗传多态性分析 ,探讨遗传因素对儿童血铅水平的影响。方法 整群抽取对铅毒性较为敏感的 6～ 10岁儿童 2 2 9名。研究两种ALAD等位基因 (ALAD1和ALAD2 )的频率并测定血铅水平 ,同时问卷调查每位研究对象血铅水平的相关因素 ,分析两种ALAD等位基因对儿童血铅水平的影响。结果 (1)所研究儿童ALAD1 1型有 2 11例 ,占 92 1% ;ALAD1 2型有 18例 ,占 7.9% ;(2 )携有等位基因ALAD2 的儿童血铅水平几何均数为 117μg/L ,携有等位基因ALAD1的儿童血铅水平几何均数为 97μg/L ;(3)儿童血铅水平相关因素分析发现 ,儿童ALAD基因型、性别、父亲职业等 9个因素与血铅显著相关。采用多元逐步回归分析 ,发现ALAD基因型对血铅水平的影响在排除协变量影响后仍有极显著的统计学意义。结论 本组儿童ALAD基因存在遗传多态性 ;携有含ALAD2
Objective Toexploretherelationshipbetweenbloodleadlevelsandthegeneticpolymorphism oftheδ aminolevulinatedehydratase (ALAD)gene .Methods TheerythrocyteALADisozymephenotypesandthe bloodleadlevelsweredeterminedinapopulationof2 2 9pre schoolchildrenandprimaryschoolstudentsaged 6 10 years.Boththeschoolandtheirhomeswerewithinonecommunitywherealargesmelterislocated .An environmentalquestionnairewasfilledforeachsubject.Results (1)ThegenedistributionoftheALADisozyme phenotypesinthesechildrenwasALAD 1 1,92 .1% ;ALAD 1 2 ,7.9% ;noALAD 2 2wasdetected .(2 )The meanbloodleadlevelsoftheenvironmentallyexposedchildrenwhowerehomozygousfortheALAD1allelewas 97μg/LwhilethemeanlevelsforthosewhowereheterozygousfortheALAD2 was 117μg/Lor 2 0 μg/Lhigher. (3)Manyfactorswerefoundtoaffectthebloodleadlevels,suchasgender,ALADisozymephenotype, educationalleveloffathersandsoon .Aftercontrollingthesepossibleconfoundingvariablesbymultipleregression , thecontributionoftheALADisozymephenotypetothebloodleadlevelswasstillstatisticallysignificant. Conclusion TheseresultsindicatethatindividualscarryingtheALAD2 allelearemorelikelytohavesustained increasesinbloodleadlevelswhenexposedtoaleadcontaminatedenvironment.Thefindingsupportsthe hypothesisthatindividualswiththeALAD2 allelemaybemoresusceptibletoleadpoisoning .
出处
《中华儿科杂志》
CSCD
北大核心
2000年第7期435-438,共4页
Chinese Journal of Pediatrics
基金
国家自然科学基金资助项目!39970645
