摘要
目的：探讨蛋白质的氧化修饰与体内抗磷脂抗体产生的关系。方法：首先，在体外以次黄嘌呤/黄嘌呤氧化酶氧化系统氧化大鼠β_2-GP1，然后以氧化的β_2-GP1（oxβ_2－GP1）免疫同种大鼠，最后以ELISA方法检测被免疫大鼠β_2-GP1血清中的抗心磷脂抗体（ACA）。结果：氧化的β_2-GP1免疫的大鼠血清中产生高滴度的ACA。结论：β_2－GP1的氧化修饰在ACA的产生上可能起十分重要的作用。
To study the relationship between the oxidaive modification of protein and the production of anti-cardiolipin antibodies. Method: Rat β_2 -GP1 was oxidized. Carhanyl groups of β_2-CP1 were detected by the reachon with 2, 4- dinitrophenylhudrazine. The rats were immunized with β_2-GP1, oxβ_2-GP1 with or without cardiolipin. ELISA was used to detect anti-cardiolipin antibodies. Result: The results showed that there were significant increase of ACA level in rat serum immunized with oxβ_2-GP1, or oxβ_2-GP1 with CL compared with that of non-immunized groups. Conclusion: The oxidative modification of β_2-GP1 is one of possible factors that induce the production of ACA.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2000年第7期359-360,共2页
Chinese Journal of Immunology
基金
国家自然科学基金!39570317
关键词
β2-糖蛋白1
抗磷脂抗体综合征
氧化修饰
ACA
β_2-glycoprotein 1 (β_2-GP1) Antiphospholipid syndrome (APS) Oxidative modification Anticardiolipin antibodies (ACA)
